1 Ocak 2013 Dergi makalesi Açık Erişim

Incesu, Zerrin; Hatipoglu, Ibrahim; Sivas, Hulya; Ergene, Emel; Ciftci, Gulsen Akalin

MARC21 XML

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <controlfield tag="001">91769</controlfield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="a">Creative Commons Attribution</subfield>
    <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="i">isVersionOf</subfield>
    <subfield code="a">10.81043/aperta.91768</subfield>
    <subfield code="n">doi</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="v">51</subfield>
    <subfield code="p">INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY</subfield>
    <subfield code="n">10</subfield>
    <subfield code="c">789-796</subfield>
  </datafield>
  <controlfield tag="005">20210316122547.0</controlfield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">user-tubitak-adresli-yayinlar</subfield>
    <subfield code="o">oai:zenodo.org:91769</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-tubitak-adresli-yayinlar</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="z">md5:2c615b661f5b1f2ad590c9d877e66c3b</subfield>
    <subfield code="s">202</subfield>
    <subfield code="u">https://aperta.ulakbim.gov.trrecord/91769/files/bib-003ec5bc-0dd4-4858-b3ab-04c205c02914.txt</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="2">doi</subfield>
    <subfield code="a">10.81043/aperta.91769</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Genet Engn &amp; Biotechnol Inst, TR-41470 Gebze, Turkey</subfield>
    <subfield code="a">Hatipoglu, Ibrahim</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey</subfield>
    <subfield code="a">Sivas, Hulya</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey</subfield>
    <subfield code="a">Ergene, Emel</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Anadolu Univ, Fac Farmacy, Dept Biochem, TR-26470 Eskisehir, Turkey</subfield>
    <subfield code="a">Ciftci, Gulsen Akalin</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">article</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2013-01-01</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">Anadolu Univ, Fac Farmacy, Dept Biochem, TR-26470 Eskisehir, Turkey</subfield>
    <subfield code="a">Incesu, Zerrin</subfield>
  </datafield>
</record>