1 Ocak 2013 Dergi makalesi Açık Erişim

Incesu, Zerrin; Hatipoglu, Ibrahim; Sivas, Hulya; Ergene, Emel; Ciftci, Gulsen Akalin

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{
  "@context": "https://schema.org/", 
  "@id": 91769, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Anadolu Univ, Fac Farmacy, Dept Biochem, TR-26470 Eskisehir, Turkey", 
      "name": "Incesu, Zerrin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Genet Engn & Biotechnol Inst, TR-41470 Gebze, Turkey", 
      "name": "Hatipoglu, Ibrahim"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey", 
      "name": "Sivas, Hulya"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey", 
      "name": "Ergene, Emel"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Anadolu Univ, Fac Farmacy, Dept Biochem, TR-26470 Eskisehir, Turkey", 
      "name": "Ciftci, Gulsen Akalin"
    }
  ], 
  "datePublished": "2013-01-01", 
  "description": "The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.", 
  "headline": "Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis", 
  "identifier": 91769, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis", 
  "url": "https://aperta.ulakbim.gov.tr/record/91769"
}