1 Ocak 2013 Dergi makalesi Açık Erişim

Incesu, Zerrin; Hatipoglu, Ibrahim; Sivas, Hulya; Ergene, Emel; Ciftci, Gulsen Akalin

Dublin Core

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Incesu, Zerrin</dc:creator>
  <dc:creator>Hatipoglu, Ibrahim</dc:creator>
  <dc:creator>Sivas, Hulya</dc:creator>
  <dc:creator>Ergene, Emel</dc:creator>
  <dc:creator>Ciftci, Gulsen Akalin</dc:creator>
  <dc:date>2013-01-01</dc:date>
  <dc:description>The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/91769</dc:identifier>
  <dc:identifier>oai:zenodo.org:91769</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY 51(10) 789-796</dc:source>
  <dc:title>Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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