1 Ocak 2013 Dergi makalesi Açık Erişim

Incesu, Zerrin; Hatipoglu, Ibrahim; Sivas, Hulya; Ergene, Emel; Ciftci, Gulsen Akalin

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{
  "URL": "https://aperta.ulakbim.gov.tr/record/91769", 
  "abstract": "The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.", 
  "author": [
    {
      "family": "Incesu", 
      "given": " Zerrin"
    }, 
    {
      "family": "Hatipoglu", 
      "given": " Ibrahim"
    }, 
    {
      "family": "Sivas", 
      "given": " Hulya"
    }, 
    {
      "family": "Ergene", 
      "given": " Emel"
    }, 
    {
      "family": "Ciftci", 
      "given": " Gulsen Akalin"
    }
  ], 
  "container_title": "INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY", 
  "id": "91769", 
  "issue": "10", 
  "issued": {
    "date-parts": [
      [
        2013, 
        1, 
        1
      ]
    ]
  }, 
  "page": "789-796", 
  "title": "Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis", 
  "type": "article-journal", 
  "volume": "51"
}