1 Ocak 2013 Dergi makalesi Açık Erişim

Incesu, Zerrin; Hatipoglu, Ibrahim; Sivas, Hulya; Ergene, Emel; Ciftci, Gulsen Akalin

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        "affiliation": "Anadolu Univ, Fac Farmacy, Dept Biochem, TR-26470 Eskisehir, Turkey", 
        "name": "Incesu, Zerrin"
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      {
        "affiliation": "Genet Engn & Biotechnol Inst, TR-41470 Gebze, Turkey", 
        "name": "Hatipoglu, Ibrahim"
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      {
        "affiliation": "Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey", 
        "name": "Sivas, Hulya"
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      {
        "affiliation": "Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey", 
        "name": "Ergene, Emel"
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      {
        "affiliation": "Anadolu Univ, Fac Farmacy, Dept Biochem, TR-26470 Eskisehir, Turkey", 
        "name": "Ciftci, Gulsen Akalin"
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    "description": "The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.", 
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      "issue": "10", 
      "pages": "789-796", 
      "title": "INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY", 
      "volume": "51"
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    "title": "Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis"
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