1 Ocak 2013 Dergi makalesi Açık Erişim

Incesu, Zerrin; Hatipoglu, Ibrahim; Sivas, Hulya; Ergene, Emel; Ciftci, Gulsen Akalin

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/91769</identifier>
  <creators>
    <creator>
      <creatorName>Incesu, Zerrin</creatorName>
      <givenName>Zerrin</givenName>
      <familyName>Incesu</familyName>
      <affiliation>Anadolu Univ, Fac Farmacy, Dept Biochem, TR-26470 Eskisehir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Hatipoglu, Ibrahim</creatorName>
      <givenName>Ibrahim</givenName>
      <familyName>Hatipoglu</familyName>
      <affiliation>Genet Engn &amp; Biotechnol Inst, TR-41470 Gebze, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sivas, Hulya</creatorName>
      <givenName>Hulya</givenName>
      <familyName>Sivas</familyName>
      <affiliation>Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ergene, Emel</creatorName>
      <givenName>Emel</givenName>
      <familyName>Ergene</familyName>
      <affiliation>Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ciftci, Gulsen Akalin</creatorName>
      <givenName>Gulsen Akalin</givenName>
      <familyName>Ciftci</familyName>
      <affiliation>Anadolu Univ, Fac Farmacy, Dept Biochem, TR-26470 Eskisehir, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Effects Of Fibronectin And Type Iv Collagen On Osteosarcoma Cell Apoptosis</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2013</publicationYear>
  <dates>
    <date dateType="Issued">2013-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/91769</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.91768</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.81043/aperta.91769</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.</description>
  </descriptions>
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