1 Ocak 2015 Dergi makalesi Açık Erişim

Kordikanlioglu, Burcu; Simsek, Omer; Saris, Per E. J.

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    <subfield code="a">Nisin Production of Lactococcus lactis N8 with Hemin-Stimulated Cell Respiration in Fed-Batch Fermentation System</subfield>
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    <subfield code="a">In this study, nisin production of Lactococcus lactis N8 was optimized by independent variables of glucose, hemin and oxygen concentrations in fed-batch fermentation in which respiration of cells was stimulated with hemin. Response surface model was able to explain the changes of the nisin production of L. lactis N8 in fed-batch fermentation system with high fidelity (R-2 98%) and insignificant lack of fit. Accordingly, the equation developed indicated the optimum parameters for glucose, hemin, and dissolved oxygen were 8 g L-1 h(-1), 3 mu g mL(-1) and 40%, respectively. While 1711 IU mL(-1) nisin was produced by L. lactis N8 in control fed-batch fermentation, 5410 IU mL(-1) nisin production was achieved within the relevant optimum parameters where the respiration of cell was stimulated with hemin. Accordingly, nisin production was enhanced 3.1 fold in fed-batch fermentation using hemin. In conclusion the nisin production of L. lactis N8 was enhanced extensively as a result of increasing the biomass by stimulating the cell respiration with adding the hemin in the fed-batch fermentation. (C) 2015 American Institute of Chemical Engineers</subfield>
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    <subfield code="u">Univ Helsinki, Dept Food &amp; Environm Sci, Helsinki, Finland</subfield>
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    <subfield code="u">Univ Pamukkale, Dept Food Engn, Denizli, Turkey</subfield>
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