1 Ocak 2015 Dergi makalesi Açık Erişim

Kordikanlioglu, Burcu; Simsek, Omer; Saris, Per E. J.

Citation Style Language JSON

{
  "DOI": "10.1002/btpr.2075", 
  "abstract": "In this study, nisin production of Lactococcus lactis N8 was optimized by independent variables of glucose, hemin and oxygen concentrations in fed-batch fermentation in which respiration of cells was stimulated with hemin. Response surface model was able to explain the changes of the nisin production of L. lactis N8 in fed-batch fermentation system with high fidelity (R-2 98%) and insignificant lack of fit. Accordingly, the equation developed indicated the optimum parameters for glucose, hemin, and dissolved oxygen were 8 g L-1 h(-1), 3 mu g mL(-1) and 40%, respectively. While 1711 IU mL(-1) nisin was produced by L. lactis N8 in control fed-batch fermentation, 5410 IU mL(-1) nisin production was achieved within the relevant optimum parameters where the respiration of cell was stimulated with hemin. Accordingly, nisin production was enhanced 3.1 fold in fed-batch fermentation using hemin. In conclusion the nisin production of L. lactis N8 was enhanced extensively as a result of increasing the biomass by stimulating the cell respiration with adding the hemin in the fed-batch fermentation. (C) 2015 American Institute of Chemical Engineers", 
  "author": [
    {
      "family": "Kordikanlioglu", 
      "given": " Burcu"
    }, 
    {
      "family": "Simsek", 
      "given": " Omer"
    }, 
    {
      "family": "Saris", 
      "given": " Per E. J."
    }
  ], 
  "container_title": "BIOTECHNOLOGY PROGRESS", 
  "id": "83197", 
  "issue": "3", 
  "issued": {
    "date-parts": [
      [
        2015, 
        1, 
        1
      ]
    ]
  }, 
  "page": "678-685", 
  "title": "Nisin Production of Lactococcus lactis N8 with Hemin-Stimulated Cell Respiration in Fed-Batch Fermentation System", 
  "type": "article-journal", 
  "volume": "31"
}