1 Ocak 2016 Dergi makalesi Açık Erişim

Asav, Engin; Sagiroglu, Ayten; Sezginturk, Mustafa Kemal

Citation Style Language JSON

{
  "DOI": "10.1002/elan.201500324", 
  "abstract": "Calretinin (CAL) is calcium binding protein, and its levels in blood and cerebrospinal fluids are increased, since its expression is increased various cancer types. A novel biosensor system fabricated by immobilization of a specific antibody to CAL, anti-Calretinin (anti-CAL), onto a gold electrode surface via an effective covalent binding method using mercaptohexanol, epichlorohydrin, and ethanolamine was reported for the sensitive, selective, and accurate analysis of CAL. The proposed biosensor showed a linear calibration range between 1 ng/mL and 5 ng/mL. LOD and LOQ values were determined as 0.11 ng/mL and 0.38 ng/mL, respectively. The standard deviation related to the reproducibility of the new biosensor system was calculated as 3.95%. Lastly, in order to state the applicability of the biosensor to early diagnosis of CAL in practice, artificial serum samples spiked with CAL have been analyzed by the proposed biosensor.", 
  "author": [
    {
      "family": "Asav", 
      "given": " Engin"
    }, 
    {
      "family": "Sagiroglu", 
      "given": " Ayten"
    }, 
    {
      "family": "Sezginturk", 
      "given": " Mustafa Kemal"
    }
  ], 
  "container_title": "ELECTROANALYSIS", 
  "id": "59215", 
  "issue": "2", 
  "issued": {
    "date-parts": [
      [
        2016, 
        1, 
        1
      ]
    ]
  }, 
  "page": "334-342", 
  "title": "Quantitative Analysis of a Promising Cancer Biomarker, Calretinin, by a Biosensing System Based on Simple and Effective Immobilization Process", 
  "type": "article-journal", 
  "volume": "28"
}