1 Ocak 2008 Dergi makalesi Açık Erişim

Karatas, S.; Mikalsen, J.; Steinum, T. M.; Taksdal, T.; Bordevik, M.; Colquhoun, D. J.

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  <dc:creator>Karatas, S.</dc:creator>
  <dc:creator>Mikalsen, J.</dc:creator>
  <dc:creator>Steinum, T. M.</dc:creator>
  <dc:creator>Taksdal, T.</dc:creator>
  <dc:creator>Bordevik, M.</dc:creator>
  <dc:creator>Colquhoun, D. J.</dc:creator>
  <dc:date>2008-01-01</dc:date>
  <dc:description>Piscirickettsia salmonis is the causative agent of piscirickettsiosis, a transmissible disease of salmonid fish. Diagnosis of piscirickettsiosis has traditionally been based upon identification of typical pathological changes by histological investigation, with confirmation by immunohistochemistry on formalin-fixed, paraffin-embedded tissues. However, implementation of more rapid confirmatory techniques, preferably with higher levels of sensitivity and possibilities for quantification, is desirable. A real-time polymerase chain reaction (PCR) assay was designed for specific detection of P. salmonis and tested on samples extracted from formalin-fixed paraffin-embedded material. Construction of a PCR-target mimic allowed determination of detection limits, linearity of the real-time PCR and quantitative detection of P. salmonis. The present study demonstrates the capability of the described real time PCR assay for detection of P. salmonis from paraffin-embedded material with a high degree of sensitivity and specificity. Implementation of this assay constitutes an important development for a rapid and secure diagnosis of piscirickettsiosis.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/38847</dc:identifier>
  <dc:identifier>oai:zenodo.org:38847</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>JOURNAL OF FISH DISEASES 31(10) 747-753</dc:source>
  <dc:title>Real time PCR detection of Piscirickettsia salmonis from formalin-fixed paraffin-embedded tissues</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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