1 Ocak 2008 Dergi makalesi Açık Erişim

Karatas, S.; Mikalsen, J.; Steinum, T. M.; Taksdal, T.; Bordevik, M.; Colquhoun, D. J.

JSON-LD (schema.org)

{
  "@context": "https://schema.org/", 
  "@id": 38847, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
      "name": "Karatas, S."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
      "name": "Mikalsen, J."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
      "name": "Steinum, T. M."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
      "name": "Taksdal, T."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Pharmaq AS, Oslo, Norway", 
      "name": "Bordevik, M."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
      "name": "Colquhoun, D. J."
    }
  ], 
  "datePublished": "2008-01-01", 
  "description": "Piscirickettsia salmonis is the causative agent of piscirickettsiosis, a transmissible disease of salmonid fish. Diagnosis of piscirickettsiosis has traditionally been based upon identification of typical pathological changes by histological investigation, with confirmation by immunohistochemistry on formalin-fixed, paraffin-embedded tissues. However, implementation of more rapid confirmatory techniques, preferably with higher levels of sensitivity and possibilities for quantification, is desirable. A real-time polymerase chain reaction (PCR) assay was designed for specific detection of P. salmonis and tested on samples extracted from formalin-fixed paraffin-embedded material. Construction of a PCR-target mimic allowed determination of detection limits, linearity of the real-time PCR and quantitative detection of P. salmonis. The present study demonstrates the capability of the described real time PCR assay for detection of P. salmonis from paraffin-embedded material with a high degree of sensitivity and specificity. Implementation of this assay constitutes an important development for a rapid and secure diagnosis of piscirickettsiosis.", 
  "headline": "Real time PCR detection of Piscirickettsia salmonis from formalin-fixed paraffin-embedded tissues", 
  "identifier": 38847, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Real time PCR detection of Piscirickettsia salmonis from formalin-fixed paraffin-embedded tissues", 
  "url": "https://aperta.ulakbim.gov.tr/record/38847"
}