1 Ocak 2008 Dergi makalesi Açık Erişim

Karatas, S.; Mikalsen, J.; Steinum, T. M.; Taksdal, T.; Bordevik, M.; Colquhoun, D. J.

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        "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
        "name": "Karatas, S."
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      {
        "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
        "name": "Mikalsen, J."
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      {
        "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
        "name": "Steinum, T. M."
      }, 
      {
        "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
        "name": "Taksdal, T."
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      {
        "affiliation": "Pharmaq AS, Oslo, Norway", 
        "name": "Bordevik, M."
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      {
        "affiliation": "Natl Vet Inst, N-0106 Oslo, Norway", 
        "name": "Colquhoun, D. J."
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    "description": "Piscirickettsia salmonis is the causative agent of piscirickettsiosis, a transmissible disease of salmonid fish. Diagnosis of piscirickettsiosis has traditionally been based upon identification of typical pathological changes by histological investigation, with confirmation by immunohistochemistry on formalin-fixed, paraffin-embedded tissues. However, implementation of more rapid confirmatory techniques, preferably with higher levels of sensitivity and possibilities for quantification, is desirable. A real-time polymerase chain reaction (PCR) assay was designed for specific detection of P. salmonis and tested on samples extracted from formalin-fixed paraffin-embedded material. Construction of a PCR-target mimic allowed determination of detection limits, linearity of the real-time PCR and quantitative detection of P. salmonis. The present study demonstrates the capability of the described real time PCR assay for detection of P. salmonis from paraffin-embedded material with a high degree of sensitivity and specificity. Implementation of this assay constitutes an important development for a rapid and secure diagnosis of piscirickettsiosis.", 
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      "issue": "10", 
      "pages": "747-753", 
      "title": "JOURNAL OF FISH DISEASES", 
      "volume": "31"
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    "title": "Real time PCR detection of Piscirickettsia salmonis from formalin-fixed paraffin-embedded tissues"
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