1 Ocak 2008 Dergi makalesi Açık Erişim

Karatas, S.; Mikalsen, J.; Steinum, T. M.; Taksdal, T.; Bordevik, M.; Colquhoun, D. J.

Citation Style Language JSON

{
  "DOI": "10.1111/j.1365-2761.2008.00948.x", 
  "abstract": "Piscirickettsia salmonis is the causative agent of piscirickettsiosis, a transmissible disease of salmonid fish. Diagnosis of piscirickettsiosis has traditionally been based upon identification of typical pathological changes by histological investigation, with confirmation by immunohistochemistry on formalin-fixed, paraffin-embedded tissues. However, implementation of more rapid confirmatory techniques, preferably with higher levels of sensitivity and possibilities for quantification, is desirable. A real-time polymerase chain reaction (PCR) assay was designed for specific detection of P. salmonis and tested on samples extracted from formalin-fixed paraffin-embedded material. Construction of a PCR-target mimic allowed determination of detection limits, linearity of the real-time PCR and quantitative detection of P. salmonis. The present study demonstrates the capability of the described real time PCR assay for detection of P. salmonis from paraffin-embedded material with a high degree of sensitivity and specificity. Implementation of this assay constitutes an important development for a rapid and secure diagnosis of piscirickettsiosis.", 
  "author": [
    {
      "family": "Karatas", 
      "given": " S."
    }, 
    {
      "family": "Mikalsen", 
      "given": " J."
    }, 
    {
      "family": "Steinum", 
      "given": " T. M."
    }, 
    {
      "family": "Taksdal", 
      "given": " T."
    }, 
    {
      "family": "Bordevik", 
      "given": " M."
    }, 
    {
      "family": "Colquhoun", 
      "given": " D. J."
    }
  ], 
  "container_title": "JOURNAL OF FISH DISEASES", 
  "id": "38847", 
  "issue": "10", 
  "issued": {
    "date-parts": [
      [
        2008, 
        1, 
        1
      ]
    ]
  }, 
  "page": "747-753", 
  "title": "Real time PCR detection of Piscirickettsia salmonis from formalin-fixed paraffin-embedded tissues", 
  "type": "article-journal", 
  "volume": "31"
}