1 Ocak 2008 Dergi makalesi Açık Erişim

Karatas, S.; Mikalsen, J.; Steinum, T. M.; Taksdal, T.; Bordevik, M.; Colquhoun, D. J.

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/38847</identifier>
  <creators>
    <creator>
      <creatorName>Karatas, S.</creatorName>
      <givenName>S.</givenName>
      <familyName>Karatas</familyName>
      <affiliation>Natl Vet Inst, N-0106 Oslo, Norway</affiliation>
    </creator>
    <creator>
      <creatorName>Mikalsen, J.</creatorName>
      <givenName>J.</givenName>
      <familyName>Mikalsen</familyName>
      <affiliation>Natl Vet Inst, N-0106 Oslo, Norway</affiliation>
    </creator>
    <creator>
      <creatorName>Steinum, T. M.</creatorName>
      <givenName>T. M.</givenName>
      <familyName>Steinum</familyName>
      <affiliation>Natl Vet Inst, N-0106 Oslo, Norway</affiliation>
    </creator>
    <creator>
      <creatorName>Taksdal, T.</creatorName>
      <givenName>T.</givenName>
      <familyName>Taksdal</familyName>
      <affiliation>Natl Vet Inst, N-0106 Oslo, Norway</affiliation>
    </creator>
    <creator>
      <creatorName>Bordevik, M.</creatorName>
      <givenName>M.</givenName>
      <familyName>Bordevik</familyName>
      <affiliation>Pharmaq AS, Oslo, Norway</affiliation>
    </creator>
    <creator>
      <creatorName>Colquhoun, D. J.</creatorName>
      <givenName>D. J.</givenName>
      <familyName>Colquhoun</familyName>
      <affiliation>Natl Vet Inst, N-0106 Oslo, Norway</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Real Time Pcr Detection Of Piscirickettsia Salmonis From Formalin-Fixed Paraffin-Embedded Tissues</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2008</publicationYear>
  <dates>
    <date dateType="Issued">2008-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/38847</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1111/j.1365-2761.2008.00948.x</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Piscirickettsia salmonis is the causative agent of piscirickettsiosis, a transmissible disease of salmonid fish. Diagnosis of piscirickettsiosis has traditionally been based upon identification of typical pathological changes by histological investigation, with confirmation by immunohistochemistry on formalin-fixed, paraffin-embedded tissues. However, implementation of more rapid confirmatory techniques, preferably with higher levels of sensitivity and possibilities for quantification, is desirable. A real-time polymerase chain reaction (PCR) assay was designed for specific detection of P. salmonis and tested on samples extracted from formalin-fixed paraffin-embedded material. Construction of a PCR-target mimic allowed determination of detection limits, linearity of the real-time PCR and quantitative detection of P. salmonis. The present study demonstrates the capability of the described real time PCR assay for detection of P. salmonis from paraffin-embedded material with a high degree of sensitivity and specificity. Implementation of this assay constitutes an important development for a rapid and secure diagnosis of piscirickettsiosis.</description>
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