1 Ocak 2021 Dergi makalesi Açık Erişim

Gozalan, Aysegul; Unaldi, Ozlem; Guldemir, Dilek; Aydogan, Sibel; Kuzucu, Cigdem; Cakirlar, Fatma Koksal; Acikgoz, Ziya Cibali; Durmaz, Riza

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{
  "DOI": "10.7883/yoken.JJID.2020.478", 
  "abstract": "We aimed to investigate the clonal relationships, common sequence types, and carbapenemase genes in 177 non-repetitive blood culture isolates of Acinetobacter baumannii collected from patients at three university hospitals in Turkey in 2016. Molecular epidemiological characteristics of the isolates were examined using pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) (Pasteur scheme-cpn60, fusA, gltA, pyrG, recA, rplB, and rpoB). Multiplex PCR was used to investigate the carbapenemase genes, including bla(OXA-23-like),bla(OXA-24-like), bla(OXA-48-like), bla(OXA-58-like), bla(IMP), bla(VIM) and bla(NDm). PFGE genotyping yielded 92 pulsotypes with a clustering ratio of 69.7%. As per a >= 85% similarity coefficient, 159 (90.9%) isolates were found to be clonally related. The bla(OXA-)(23-like) and bla(OXA-)(58)-like genes were identified in 100% and 28.2% of the isolates, respectively. The bla(NDM) gene was identified in two isolates. The MLST analysis included 54 isolates with different pulsotypes, and 29 sequence types (STs). Most of the isolates (n = 36) belonged to the clonal complex (CC)2, one isolate belonged to CC1, and one isolate belonged to CC164. Sixteen new STs (ST1235-ST1250) were identified. Identifying both global ST2 and a large number of new STs, revealed high genetic diversity in A. baumannii isolates in the study population.", 
  "author": [
    {
      "family": "Gozalan", 
      "given": " Aysegul"
    }, 
    {
      "family": "Unaldi", 
      "given": " Ozlem"
    }, 
    {
      "family": "Guldemir", 
      "given": " Dilek"
    }, 
    {
      "family": "Aydogan", 
      "given": " Sibel"
    }, 
    {
      "family": "Kuzucu", 
      "given": " Cigdem"
    }, 
    {
      "family": "Cakirlar", 
      "given": " Fatma Koksal"
    }, 
    {
      "family": "Acikgoz", 
      "given": " Ziya Cibali"
    }, 
    {
      "family": "Durmaz", 
      "given": " Riza"
    }
  ], 
  "container_title": "JAPANESE JOURNAL OF INFECTIOUS DISEASES", 
  "id": "233202", 
  "issue": "3", 
  "issued": {
    "date-parts": [
      [
        2021, 
        1, 
        1
      ]
    ]
  }, 
  "page": "200-208", 
  "title": "Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii Blood Culture Isolates from Three Hospitals in Turkey", 
  "type": "article-journal", 
  "volume": "74"
}