1 Ocak 1996 Dergi makalesi Açık Erişim

Mustafaev, M; Yucel, F; Cirakoglu, B; Bermek, E

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Mustafaev, M</dc:creator>
  <dc:creator>Yucel, F</dc:creator>
  <dc:creator>Cirakoglu, B</dc:creator>
  <dc:creator>Bermek, E</dc:creator>
  <dc:date>1996-01-01</dc:date>
  <dc:description>The immunogenic properties of water soluble (PAA-Cu2+-BSA) and colloidal (PAA-Cu2+-BSA.P) polycomplexes were investigated, and the specificity of antibodies produced was analyzed. Polycomplexes containing progesterone appeared to possess a high steroid-specific immunogenic activity. A comparative study of immunogenic properties of polycomplexes versus BSA.P + incomplete Freund's adjuvant (IFA) mixtures revealed differences in regards to the specificity of antibody production. In contrast to the IFA system, polycomplexes were able to generate P- as well as BSA-specific antibodies. Such a response is determined, possibly, by increases in the immunogenicity of weak antigenic determinants on the surface of protein globules and/or by the representation of 'dormant' determinants existing in the inner site upon complex formation with polyelectrolytes. Finally, using a short immunization procedure based on use of PAA-Cu2+-BSA polycomplexes, we produced seven monoclonal antibodies against progesterone included in polyelectrolyte complexes with affinities K-d ranging between 1.3 x 10(-7) and 9 x 10(-8) M.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/103801</dc:identifier>
  <dc:identifier>oai:zenodo.org:103801</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>IMMUNOLOGY LETTERS 52(2-3) 63-68</dc:source>
  <dc:title>Immune response to progesterone involved in Cu2+-mediated polyanion-protein complex - Antigen specificity and affinity of hybridoma clones</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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