1 Ocak 1996 Dergi makalesi Açık Erişim

Mustafaev, M; Yucel, F; Cirakoglu, B; Bermek, E

Citation Style Language JSON

{
  "URL": "https://aperta.ulakbim.gov.tr/record/103801", 
  "abstract": "The immunogenic properties of water soluble (PAA-Cu2+-BSA) and colloidal (PAA-Cu2+-BSA.P) polycomplexes were investigated, and the specificity of antibodies produced was analyzed. Polycomplexes containing progesterone appeared to possess a high steroid-specific immunogenic activity. A comparative study of immunogenic properties of polycomplexes versus BSA.P + incomplete Freund's adjuvant (IFA) mixtures revealed differences in regards to the specificity of antibody production. In contrast to the IFA system, polycomplexes were able to generate P- as well as BSA-specific antibodies. Such a response is determined, possibly, by increases in the immunogenicity of weak antigenic determinants on the surface of protein globules and/or by the representation of 'dormant' determinants existing in the inner site upon complex formation with polyelectrolytes. Finally, using a short immunization procedure based on use of PAA-Cu2+-BSA polycomplexes, we produced seven monoclonal antibodies against progesterone included in polyelectrolyte complexes with affinities K-d ranging between 1.3 x 10(-7) and 9 x 10(-8) M.", 
  "author": [
    {
      "family": "Mustafaev", 
      "given": " M"
    }, 
    {
      "family": "Yucel", 
      "given": " F"
    }, 
    {
      "family": "Cirakoglu", 
      "given": " B"
    }, 
    {
      "family": "Bermek", 
      "given": " E"
    }
  ], 
  "container_title": "IMMUNOLOGY LETTERS", 
  "id": "103801", 
  "issue": "2-3", 
  "issued": {
    "date-parts": [
      [
        1996, 
        1, 
        1
      ]
    ]
  }, 
  "page": "63-68", 
  "title": "Immune response to progesterone involved in Cu2+-mediated polyanion-protein complex - Antigen specificity and affinity of hybridoma clones", 
  "type": "article-journal", 
  "volume": "52"
}