1 Ocak 1996 Dergi makalesi Açık Erişim

Mustafaev, M; Yucel, F; Cirakoglu, B; Bermek, E

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{
  "@context": "https://schema.org/", 
  "@id": 103801, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Mustafaev, M"
    }, 
    {
      "@type": "Person", 
      "name": "Yucel, F"
    }, 
    {
      "@type": "Person", 
      "name": "Cirakoglu, B"
    }, 
    {
      "@type": "Person", 
      "name": "Bermek, E"
    }
  ], 
  "datePublished": "1996-01-01", 
  "description": "The immunogenic properties of water soluble (PAA-Cu2+-BSA) and colloidal (PAA-Cu2+-BSA.P) polycomplexes were investigated, and the specificity of antibodies produced was analyzed. Polycomplexes containing progesterone appeared to possess a high steroid-specific immunogenic activity. A comparative study of immunogenic properties of polycomplexes versus BSA.P + incomplete Freund's adjuvant (IFA) mixtures revealed differences in regards to the specificity of antibody production. In contrast to the IFA system, polycomplexes were able to generate P- as well as BSA-specific antibodies. Such a response is determined, possibly, by increases in the immunogenicity of weak antigenic determinants on the surface of protein globules and/or by the representation of 'dormant' determinants existing in the inner site upon complex formation with polyelectrolytes. Finally, using a short immunization procedure based on use of PAA-Cu2+-BSA polycomplexes, we produced seven monoclonal antibodies against progesterone included in polyelectrolyte complexes with affinities K-d ranging between 1.3 x 10(-7) and 9 x 10(-8) M.", 
  "headline": "Immune response to progesterone involved in Cu2+-mediated polyanion-protein complex - Antigen specificity and affinity of hybridoma clones", 
  "identifier": 103801, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Immune response to progesterone involved in Cu2+-mediated polyanion-protein complex - Antigen specificity and affinity of hybridoma clones", 
  "url": "https://aperta.ulakbim.gov.tr/record/103801"
}