1 Ocak 1996 Dergi makalesi Açık Erişim

Mustafaev, M; Yucel, F; Cirakoglu, B; Bermek, E

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/103801</identifier>
  <creators>
    <creator>
      <creatorName>Mustafaev, M</creatorName>
      <givenName>M</givenName>
      <familyName>Mustafaev</familyName>
    </creator>
    <creator>
      <creatorName>Yucel, F</creatorName>
      <givenName>F</givenName>
      <familyName>Yucel</familyName>
    </creator>
    <creator>
      <creatorName>Cirakoglu, B</creatorName>
      <givenName>B</givenName>
      <familyName>Cirakoglu</familyName>
    </creator>
    <creator>
      <creatorName>Bermek, E</creatorName>
      <givenName>E</givenName>
      <familyName>Bermek</familyName>
    </creator>
  </creators>
  <titles>
    <title>Immune Response To Progesterone Involved In Cu2+-Mediated Polyanion-Protein Complex - Antigen Specificity And Affinity Of Hybridoma Clones</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>1996</publicationYear>
  <dates>
    <date dateType="Issued">1996-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/103801</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.103800</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.81043/aperta.103801</relatedIdentifier>
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  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">The immunogenic properties of water soluble (PAA-Cu2+-BSA) and colloidal (PAA-Cu2+-BSA.P) polycomplexes were investigated, and the specificity of antibodies produced was analyzed. Polycomplexes containing progesterone appeared to possess a high steroid-specific immunogenic activity. A comparative study of immunogenic properties of polycomplexes versus BSA.P + incomplete Freund's adjuvant (IFA) mixtures revealed differences in regards to the specificity of antibody production. In contrast to the IFA system, polycomplexes were able to generate P- as well as BSA-specific antibodies. Such a response is determined, possibly, by increases in the immunogenicity of weak antigenic determinants on the surface of protein globules and/or by the representation of 'dormant' determinants existing in the inner site upon complex formation with polyelectrolytes. Finally, using a short immunization procedure based on use of PAA-Cu2+-BSA polycomplexes, we produced seven monoclonal antibodies against progesterone included in polyelectrolyte complexes with affinities K-d ranging between 1.3 x 10(-7) and 9 x 10(-8) M.</description>
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