1 Ocak 2000 Dergi makalesi Açık Erişim

Basalp, A; Mustafaeva, Z; Mustafaev, M; Bermek, E

Dublin Core

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  <dc:creator>Basalp, A</dc:creator>
  <dc:creator>Mustafaeva, Z</dc:creator>
  <dc:creator>Mustafaev, M</dc:creator>
  <dc:creator>Bermek, E</dc:creator>
  <dc:date>2000-01-01</dc:date>
  <dc:description>The immunogenic properties of 17 beta -estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17 beta -estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with K-d values ranging between 1.2 X 10(-7) and 8 X 10(-8) M.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/95361</dc:identifier>
  <dc:identifier>oai:zenodo.org:95361</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>HYBRIDOMA 19(6) 495-499</dc:source>
  <dc:title>Immune response to 17 beta-estradiol involved in polymer gels: Antigen specificity and affinity of hybridoma clones</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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