1 Ocak 2000 Dergi makalesi Açık Erişim

Basalp, A; Mustafaeva, Z; Mustafaev, M; Bermek, E

JSON

{
  "conceptdoi": "10.81043/aperta.95360", 
  "conceptrecid": "95360", 
  "created": "2021-03-16T13:14:52.748900+00:00", 
  "doi": "10.81043/aperta.95361", 
  "files": [
    {
      "bucket": "a09a3800-711c-4570-84b7-c0c8118d55ce", 
      "checksum": "md5:b949a37bd8f4e70a61c9b168e905a902", 
      "key": "bib-a97e355c-e1bf-45a1-bb59-8683834b66e5.txt", 
      "links": {
        "self": "https://aperta.ulakbim.gov.tr/api/files/a09a3800-711c-4570-84b7-c0c8118d55ce/bib-a97e355c-e1bf-45a1-bb59-8683834b66e5.txt"
      }, 
      "size": 206, 
      "type": "txt"
    }
  ], 
  "id": 95361, 
  "links": {
    "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.81043/aperta.95361.svg", 
    "bucket": "https://aperta.ulakbim.gov.tr/api/files/a09a3800-711c-4570-84b7-c0c8118d55ce", 
    "conceptbadge": "https://aperta.ulakbim.gov.tr/badge/doi/10.81043/aperta.95360.svg", 
    "conceptdoi": "https://doi.org/10.81043/aperta.95360", 
    "doi": "https://doi.org/10.81043/aperta.95361", 
    "html": "https://aperta.ulakbim.gov.tr/record/95361", 
    "latest": "https://aperta.ulakbim.gov.tr/api/records/95361", 
    "latest_html": "https://aperta.ulakbim.gov.tr/record/95361"
  }, 
  "metadata": {
    "access_right": "open", 
    "access_right_category": "success", 
    "communities": [
      {
        "id": "tubitak-adresli-yayinlar"
      }
    ], 
    "creators": [
      {
        "name": "Basalp, A"
      }, 
      {
        "name": "Mustafaeva, Z"
      }, 
      {
        "name": "Mustafaev, M"
      }, 
      {
        "name": "Bermek, E"
      }
    ], 
    "description": "The immunogenic properties of 17 beta -estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17 beta -estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with K-d values ranging between 1.2 X 10(-7) and 8 X 10(-8) M.", 
    "doi": "10.81043/aperta.95361", 
    "has_grant": false, 
    "journal": {
      "issue": "6", 
      "pages": "495-499", 
      "title": "HYBRIDOMA", 
      "volume": "19"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2000-01-01", 
    "related_identifiers": [
      {
        "identifier": "10.81043/aperta.95360", 
        "relation": "isVersionOf", 
        "scheme": "doi"
      }
    ], 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "95361"
          }, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "95360"
          }
        }
      ]
    }, 
    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
    }, 
    "title": "Immune response to 17 beta-estradiol involved in polymer gels: Antigen specificity and affinity of hybridoma clones"
  }, 
  "owners": [
    1
  ], 
  "revision": 1, 
  "stats": {
    "downloads": 5.0, 
    "unique_downloads": 5.0, 
    "unique_views": 12.0, 
    "version_downloads": 5.0, 
    "version_unique_downloads": 5.0, 
    "version_unique_views": 12.0, 
    "version_views": 12.0, 
    "version_volume": 1030.0, 
    "views": 12.0, 
    "volume": 1030.0
  }, 
  "updated": "2021-03-16T13:14:52.804966+00:00"
}