1 Ocak 2000 Dergi makalesi Açık Erişim

Basalp, A; Mustafaeva, Z; Mustafaev, M; Bermek, E

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{
  "URL": "https://aperta.ulakbim.gov.tr/record/95361", 
  "abstract": "The immunogenic properties of 17 beta -estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17 beta -estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with K-d values ranging between 1.2 X 10(-7) and 8 X 10(-8) M.", 
  "author": [
    {
      "family": "Basalp", 
      "given": " A"
    }, 
    {
      "family": "Mustafaeva", 
      "given": " Z"
    }, 
    {
      "family": "Mustafaev", 
      "given": " M"
    }, 
    {
      "family": "Bermek", 
      "given": " E"
    }
  ], 
  "container_title": "HYBRIDOMA", 
  "id": "95361", 
  "issue": "6", 
  "issued": {
    "date-parts": [
      [
        2000, 
        1, 
        1
      ]
    ]
  }, 
  "page": "495-499", 
  "title": "Immune response to 17 beta-estradiol involved in polymer gels: Antigen specificity and affinity of hybridoma clones", 
  "type": "article-journal", 
  "volume": "19"
}