1 Ocak 2000 Dergi makalesi Açık Erişim

Basalp, A; Mustafaeva, Z; Mustafaev, M; Bermek, E

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/95361</identifier>
  <creators>
    <creator>
      <creatorName>Basalp, A</creatorName>
      <givenName>A</givenName>
      <familyName>Basalp</familyName>
    </creator>
    <creator>
      <creatorName>Mustafaeva, Z</creatorName>
      <givenName>Z</givenName>
      <familyName>Mustafaeva</familyName>
    </creator>
    <creator>
      <creatorName>Mustafaev, M</creatorName>
      <givenName>M</givenName>
      <familyName>Mustafaev</familyName>
    </creator>
    <creator>
      <creatorName>Bermek, E</creatorName>
      <givenName>E</givenName>
      <familyName>Bermek</familyName>
    </creator>
  </creators>
  <titles>
    <title>Immune Response To 17 Beta-Estradiol Involved In Polymer Gels: Antigen Specificity And Affinity Of Hybridoma Clones</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2000</publicationYear>
  <dates>
    <date dateType="Issued">2000-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/95361</alternateIdentifier>
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  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.95360</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.81043/aperta.95361</relatedIdentifier>
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  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
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  <descriptions>
    <description descriptionType="Abstract">The immunogenic properties of 17 beta -estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17 beta -estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with K-d values ranging between 1.2 X 10(-7) and 8 X 10(-8) M.</description>
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