1 Ocak 2018 Dergi makalesi Açık Erişim

Akkoc, Tunc; Genc, Deniz; Zibandeh, Noushin; Akkoc, Tolga

Dublin Core

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Akkoc, Tunc</dc:creator>
  <dc:creator>Genc, Deniz</dc:creator>
  <dc:creator>Zibandeh, Noushin</dc:creator>
  <dc:creator>Akkoc, Tolga</dc:creator>
  <dc:date>2018-01-01</dc:date>
  <dc:description>Allergen-specific immunotherapy to induce T regulatory cells in the periphery has been used to treat allergic diseases. Mycobacteria can be used as an adjuvant for inducing T regulatory cells. However, it is unclear whether intranasal immunotherapy in combination with Mycobacteria adjuvant induces regulatory T cell differentiation and attenuates allergic responses in vivo. To investigate the role of intranasal ovalbumin (OVA) treatment alone and in combination with Mycobacteria vaccae, proportions of FoxP3(+) regulatory T cells and anti-inflammatory responses were evaluated in a murine model of asthma that was established in three groups of bicistronic Foxp3(EGFP) reporter BALB/c mice. Before establishment of the asthma model, two groups of mice received intranasal OVA immunotherapy and one also received simultaneous s.c. M. vaccae. Expression of CD4(+)CD25(+)Foxp3(+EGFP+) T cells in the lung and spleen was analyzed by flow cytometry and the cytokine profiles of allergen-stimulated lung and spleen lymphocytes assessed. The intranasal OVA immunotherapy group showed greater expression of CD4(+)CD25(+)Foxp3(+EGFP+) T cells in the spleen whereas in the group that also received M. vaccae such greater expression was demonstrated in the lung. Additionally, the proportion of IL-10 and IFN--secreting splenocytes was greater in the intranasal OVA+M. vaccae group. CD25 neutralization decreased CD4(+)Foxp3(+) cells more than other groups. In parallel with this finding, production of IL-10 and IFN- was down-regulated. Mucosal administration of OVA antigen results in a greater proportion of CD4(+)Foxp3(+) T cells in the spleen. IL-10 and IFN- induced by intranasal OVA immunotherapy and M. vaccae administration is down-regulated after CD25 neutralization.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/89813</dc:identifier>
  <dc:identifier>oai:zenodo.org:89813</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>MICROBIOLOGY AND IMMUNOLOGY 62(8) 531-540</dc:source>
  <dc:title>Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
</oai_dc:dc>