1 Ocak 2018 Dergi makalesi Açık Erişim

Akkoc, Tunc; Genc, Deniz; Zibandeh, Noushin; Akkoc, Tolga

Citation Style Language JSON

{
  "DOI": "10.1111/1348-0421.12634", 
  "abstract": "Allergen-specific immunotherapy to induce T regulatory cells in the periphery has been used to treat allergic diseases. Mycobacteria can be used as an adjuvant for inducing T regulatory cells. However, it is unclear whether intranasal immunotherapy in combination with Mycobacteria adjuvant induces regulatory T cell differentiation and attenuates allergic responses in vivo. To investigate the role of intranasal ovalbumin (OVA) treatment alone and in combination with Mycobacteria vaccae, proportions of FoxP3(+) regulatory T cells and anti-inflammatory responses were evaluated in a murine model of asthma that was established in three groups of bicistronic Foxp3(EGFP) reporter BALB/c mice. Before establishment of the asthma model, two groups of mice received intranasal OVA immunotherapy and one also received simultaneous s.c. M. vaccae. Expression of CD4(+)CD25(+)Foxp3(+EGFP+) T cells in the lung and spleen was analyzed by flow cytometry and the cytokine profiles of allergen-stimulated lung and spleen lymphocytes assessed. The intranasal OVA immunotherapy group showed greater expression of CD4(+)CD25(+)Foxp3(+EGFP+) T cells in the spleen whereas in the group that also received M. vaccae such greater expression was demonstrated in the lung. Additionally, the proportion of IL-10 and IFN--secreting splenocytes was greater in the intranasal OVA+M. vaccae group. CD25 neutralization decreased CD4(+)Foxp3(+) cells more than other groups. In parallel with this finding, production of IL-10 and IFN- was down-regulated. Mucosal administration of OVA antigen results in a greater proportion of CD4(+)Foxp3(+) T cells in the spleen. IL-10 and IFN- induced by intranasal OVA immunotherapy and M. vaccae administration is down-regulated after CD25 neutralization.", 
  "author": [
    {
      "family": "Akkoc", 
      "given": " Tunc"
    }, 
    {
      "family": "Genc", 
      "given": " Deniz"
    }, 
    {
      "family": "Zibandeh", 
      "given": " Noushin"
    }, 
    {
      "family": "Akkoc", 
      "given": " Tolga"
    }
  ], 
  "container_title": "MICROBIOLOGY AND IMMUNOLOGY", 
  "id": "89813", 
  "issue": "8", 
  "issued": {
    "date-parts": [
      [
        2018, 
        1, 
        1
      ]
    ]
  }, 
  "page": "531-540", 
  "title": "Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues", 
  "type": "article-journal", 
  "volume": "62"
}