1 Ocak 2018 Dergi makalesi Açık Erişim

Akkoc, Tunc; Genc, Deniz; Zibandeh, Noushin; Akkoc, Tolga

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{
  "@context": "https://schema.org/", 
  "@id": 89813, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Marmara Univ, Div Pediat Allergy & Immunol, Fac Med, Istanbul, Turkey", 
      "name": "Akkoc, Tunc"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Marmara Univ, Div Pediat Allergy & Immunol, Fac Med, Istanbul, Turkey", 
      "name": "Genc, Deniz"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Marmara Univ, Div Pediat Allergy & Immunol, Fac Med, Istanbul, Turkey", 
      "name": "Zibandeh, Noushin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Sci & Technol Res Council Turkey, Marmara Res Ctr, Kocaeli, Turkey", 
      "name": "Akkoc, Tolga"
    }
  ], 
  "datePublished": "2018-01-01", 
  "description": "Allergen-specific immunotherapy to induce T regulatory cells in the periphery has been used to treat allergic diseases. Mycobacteria can be used as an adjuvant for inducing T regulatory cells. However, it is unclear whether intranasal immunotherapy in combination with Mycobacteria adjuvant induces regulatory T cell differentiation and attenuates allergic responses in vivo. To investigate the role of intranasal ovalbumin (OVA) treatment alone and in combination with Mycobacteria vaccae, proportions of FoxP3(+) regulatory T cells and anti-inflammatory responses were evaluated in a murine model of asthma that was established in three groups of bicistronic Foxp3(EGFP) reporter BALB/c mice. Before establishment of the asthma model, two groups of mice received intranasal OVA immunotherapy and one also received simultaneous s.c. M. vaccae. Expression of CD4(+)CD25(+)Foxp3(+EGFP+) T cells in the lung and spleen was analyzed by flow cytometry and the cytokine profiles of allergen-stimulated lung and spleen lymphocytes assessed. The intranasal OVA immunotherapy group showed greater expression of CD4(+)CD25(+)Foxp3(+EGFP+) T cells in the spleen whereas in the group that also received M. vaccae such greater expression was demonstrated in the lung. Additionally, the proportion of IL-10 and IFN--secreting splenocytes was greater in the intranasal OVA+M. vaccae group. CD25 neutralization decreased CD4(+)Foxp3(+) cells more than other groups. In parallel with this finding, production of IL-10 and IFN- was down-regulated. Mucosal administration of OVA antigen results in a greater proportion of CD4(+)Foxp3(+) T cells in the spleen. IL-10 and IFN- induced by intranasal OVA immunotherapy and M. vaccae administration is down-regulated after CD25 neutralization.", 
  "headline": "Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues", 
  "identifier": 89813, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues", 
  "url": "https://aperta.ulakbim.gov.tr/record/89813"
}