1 Ocak 2018 Dergi makalesi Açık Erişim

Akkoc, Tunc; Genc, Deniz; Zibandeh, Noushin; Akkoc, Tolga

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/89813</identifier>
  <creators>
    <creator>
      <creatorName>Akkoc, Tunc</creatorName>
      <givenName>Tunc</givenName>
      <familyName>Akkoc</familyName>
      <affiliation>Marmara Univ, Div Pediat Allergy &amp; Immunol, Fac Med, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Genc, Deniz</creatorName>
      <givenName>Deniz</givenName>
      <familyName>Genc</familyName>
      <affiliation>Marmara Univ, Div Pediat Allergy &amp; Immunol, Fac Med, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Zibandeh, Noushin</creatorName>
      <givenName>Noushin</givenName>
      <familyName>Zibandeh</familyName>
      <affiliation>Marmara Univ, Div Pediat Allergy &amp; Immunol, Fac Med, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Akkoc, Tolga</creatorName>
      <givenName>Tolga</givenName>
      <familyName>Akkoc</familyName>
      <affiliation>Sci &amp; Technol Res Council Turkey, Marmara Res Ctr, Kocaeli, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Intranasal Ovalbumin Immunotherapy With Mycobacterial Adjuvant Promotes Regulatory T Cell Accumulation In Lung Tissues</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2018</publicationYear>
  <dates>
    <date dateType="Issued">2018-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/89813</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1111/1348-0421.12634</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Allergen-specific immunotherapy to induce T regulatory cells in the periphery has been used to treat allergic diseases. Mycobacteria can be used as an adjuvant for inducing T regulatory cells. However, it is unclear whether intranasal immunotherapy in combination with Mycobacteria adjuvant induces regulatory T cell differentiation and attenuates allergic responses in vivo. To investigate the role of intranasal ovalbumin (OVA) treatment alone and in combination with Mycobacteria vaccae, proportions of FoxP3(+) regulatory T cells and anti-inflammatory responses were evaluated in a murine model of asthma that was established in three groups of bicistronic Foxp3(EGFP) reporter BALB/c mice. Before establishment of the asthma model, two groups of mice received intranasal OVA immunotherapy and one also received simultaneous s.c. M. vaccae. Expression of CD4(+)CD25(+)Foxp3(+EGFP+) T cells in the lung and spleen was analyzed by flow cytometry and the cytokine profiles of allergen-stimulated lung and spleen lymphocytes assessed. The intranasal OVA immunotherapy group showed greater expression of CD4(+)CD25(+)Foxp3(+EGFP+) T cells in the spleen whereas in the group that also received M. vaccae such greater expression was demonstrated in the lung. Additionally, the proportion of IL-10 and IFN--secreting splenocytes was greater in the intranasal OVA+M. vaccae group. CD25 neutralization decreased CD4(+)Foxp3(+) cells more than other groups. In parallel with this finding, production of IL-10 and IFN- was down-regulated. Mucosal administration of OVA antigen results in a greater proportion of CD4(+)Foxp3(+) T cells in the spleen. IL-10 and IFN- induced by intranasal OVA immunotherapy and M. vaccae administration is down-regulated after CD25 neutralization.</description>
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