1 Ocak 2019 Dergi makalesi Açık Erişim

Ispirli, Humeyra; Yuzer, Mustafa Onur; Skory, Christopher; Colquhoun, Ian J.; Sagdic, Osman; Dertli, Enes

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    <subfield code="a">Glucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of alpha-glucans with different structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified and heterologously expressed. Alignments of GTFA with other glucansucrases revealed its novelty and a putative 3D model structure was obtained. The biochemical properties of the truncated enzyme without the N-terminal variable region, GTFA-Delta N, was characterized. The K-m and V-max were found to be 7.5 mM and 1.49 IU/mg, respectively, and it showed optimum activities at pH 7 and at 50 degrees C. The GTFA-Delta N produced in vitro an alpha-glucan with (alpha 1 -&amp;gt; 3) and (alpha 1 -&amp;gt; 6) glycosidic linkages using sucrose as the substrate. Importantly, GTFA-Delta N synthesized DP = 9 oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively, as detected by TLC, HPLC, LC-MS and NMR analysis.</subfield>
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    <subfield code="a">10.1080/10242422.2019.1593969</subfield>
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    <subfield code="a">Characterization of a glucansucrase from Lactobacillus reuteri E81 and production of malto-oligosaccharides</subfield>
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    <subfield code="a">Yuzer, Mustafa Onur</subfield>
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