1 Ocak 2019 Dergi makalesi Açık Erişim

Ispirli, Humeyra; Yuzer, Mustafa Onur; Skory, Christopher; Colquhoun, Ian J.; Sagdic, Osman; Dertli, Enes

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/71257</identifier>
  <creators>
    <creator>
      <creatorName>Ispirli, Humeyra</creatorName>
      <givenName>Humeyra</givenName>
      <familyName>Ispirli</familyName>
      <affiliation>Yildiz Tech Univ, Chem &amp; Met Engn Fac, Dept Food Engn, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yuzer, Mustafa Onur</creatorName>
      <givenName>Mustafa Onur</givenName>
      <familyName>Yuzer</familyName>
      <affiliation>Bayburt Univ, Fac Engn, Dept Food Engn, TR-69000 Bayburt, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Skory, Christopher</creatorName>
      <givenName>Christopher</givenName>
      <familyName>Skory</familyName>
      <affiliation>Natl Ctr Agr Utilizat Res Agr Res Serv, Dept Agr, Renewable Prod Technol Res Unit, Peoria, IL USA</affiliation>
    </creator>
    <creator>
      <creatorName>Colquhoun, Ian J.</creatorName>
      <givenName>Ian J.</givenName>
      <familyName>Colquhoun</familyName>
      <affiliation>Quadram Inst Biosci, Analyt Sci Unit, Norwich, Norfolk, England</affiliation>
    </creator>
    <creator>
      <creatorName>Sagdic, Osman</creatorName>
      <givenName>Osman</givenName>
      <familyName>Sagdic</familyName>
      <affiliation>Yildiz Tech Univ, Chem &amp; Met Engn Fac, Dept Food Engn, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Dertli, Enes</creatorName>
      <givenName>Enes</givenName>
      <familyName>Dertli</familyName>
      <affiliation>Bayburt Univ, Fac Engn, Dept Food Engn, TR-69000 Bayburt, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Characterization Of A Glucansucrase From Lactobacillus Reuteri E81 And Production Of Malto-Oligosaccharides</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2019</publicationYear>
  <dates>
    <date dateType="Issued">2019-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/71257</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1080/10242422.2019.1593969</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Glucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of alpha-glucans with different structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified and heterologously expressed. Alignments of GTFA with other glucansucrases revealed its novelty and a putative 3D model structure was obtained. The biochemical properties of the truncated enzyme without the N-terminal variable region, GTFA-Delta N, was characterized. The K-m and V-max were found to be 7.5 mM and 1.49 IU/mg, respectively, and it showed optimum activities at pH 7 and at 50 degrees C. The GTFA-Delta N produced in vitro an alpha-glucan with (alpha 1 -&amp;gt; 3) and (alpha 1 -&amp;gt; 6) glycosidic linkages using sucrose as the substrate. Importantly, GTFA-Delta N synthesized DP = 9 oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively, as detected by TLC, HPLC, LC-MS and NMR analysis.</description>
  </descriptions>
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