1 Ocak 2019 Dergi makalesi Açık Erişim

Ispirli, Humeyra; Yuzer, Mustafa Onur; Skory, Christopher; Colquhoun, Ian J.; Sagdic, Osman; Dertli, Enes

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{
  "@context": "https://schema.org/", 
  "@id": 71257, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Yildiz Tech Univ, Chem & Met Engn Fac, Dept Food Engn, Istanbul, Turkey", 
      "name": "Ispirli, Humeyra"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bayburt Univ, Fac Engn, Dept Food Engn, TR-69000 Bayburt, Turkey", 
      "name": "Yuzer, Mustafa Onur"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Natl Ctr Agr Utilizat Res Agr Res Serv, Dept Agr, Renewable Prod Technol Res Unit, Peoria, IL USA", 
      "name": "Skory, Christopher"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Quadram Inst Biosci, Analyt Sci Unit, Norwich, Norfolk, England", 
      "name": "Colquhoun, Ian J."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yildiz Tech Univ, Chem & Met Engn Fac, Dept Food Engn, Istanbul, Turkey", 
      "name": "Sagdic, Osman"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bayburt Univ, Fac Engn, Dept Food Engn, TR-69000 Bayburt, Turkey", 
      "name": "Dertli, Enes"
    }
  ], 
  "datePublished": "2019-01-01", 
  "description": "Glucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of alpha-glucans with different structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified and heterologously expressed. Alignments of GTFA with other glucansucrases revealed its novelty and a putative 3D model structure was obtained. The biochemical properties of the truncated enzyme without the N-terminal variable region, GTFA-Delta N, was characterized. The K-m and V-max were found to be 7.5 mM and 1.49 IU/mg, respectively, and it showed optimum activities at pH 7 and at 50 degrees C. The GTFA-Delta N produced in vitro an alpha-glucan with (alpha 1 -> 3) and (alpha 1 -> 6) glycosidic linkages using sucrose as the substrate. Importantly, GTFA-Delta N synthesized DP = 9 oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively, as detected by TLC, HPLC, LC-MS and NMR analysis.", 
  "headline": "Characterization of a glucansucrase from Lactobacillus reuteri E81 and production of malto-oligosaccharides", 
  "identifier": 71257, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Characterization of a glucansucrase from Lactobacillus reuteri E81 and production of malto-oligosaccharides", 
  "url": "https://aperta.ulakbim.gov.tr/record/71257"
}