1 Ocak 2019 Dergi makalesi Açık Erişim

Ispirli, Humeyra; Yuzer, Mustafa Onur; Skory, Christopher; Colquhoun, Ian J.; Sagdic, Osman; Dertli, Enes

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Ispirli, Humeyra</dc:creator>
  <dc:creator>Yuzer, Mustafa Onur</dc:creator>
  <dc:creator>Skory, Christopher</dc:creator>
  <dc:creator>Colquhoun, Ian J.</dc:creator>
  <dc:creator>Sagdic, Osman</dc:creator>
  <dc:creator>Dertli, Enes</dc:creator>
  <dc:date>2019-01-01</dc:date>
  <dc:description>Glucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of alpha-glucans with different structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified and heterologously expressed. Alignments of GTFA with other glucansucrases revealed its novelty and a putative 3D model structure was obtained. The biochemical properties of the truncated enzyme without the N-terminal variable region, GTFA-Delta N, was characterized. The K-m and V-max were found to be 7.5 mM and 1.49 IU/mg, respectively, and it showed optimum activities at pH 7 and at 50 degrees C. The GTFA-Delta N produced in vitro an alpha-glucan with (alpha 1 -&gt; 3) and (alpha 1 -&gt; 6) glycosidic linkages using sucrose as the substrate. Importantly, GTFA-Delta N synthesized DP = 9 oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively, as detected by TLC, HPLC, LC-MS and NMR analysis.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/71257</dc:identifier>
  <dc:identifier>oai:zenodo.org:71257</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>BIOCATALYSIS AND BIOTRANSFORMATION 37(6) 421-430</dc:source>
  <dc:title>Characterization of a glucansucrase from Lactobacillus reuteri E81 and production of malto-oligosaccharides</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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