1 Ocak 2019 Dergi makalesi Açık Erişim

Ispirli, Humeyra; Yuzer, Mustafa Onur; Skory, Christopher; Colquhoun, Ian J.; Sagdic, Osman; Dertli, Enes

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{
  "DOI": "10.1080/10242422.2019.1593969", 
  "abstract": "Glucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of alpha-glucans with different structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified and heterologously expressed. Alignments of GTFA with other glucansucrases revealed its novelty and a putative 3D model structure was obtained. The biochemical properties of the truncated enzyme without the N-terminal variable region, GTFA-Delta N, was characterized. The K-m and V-max were found to be 7.5 mM and 1.49 IU/mg, respectively, and it showed optimum activities at pH 7 and at 50 degrees C. The GTFA-Delta N produced in vitro an alpha-glucan with (alpha 1 -> 3) and (alpha 1 -> 6) glycosidic linkages using sucrose as the substrate. Importantly, GTFA-Delta N synthesized DP = 9 oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively, as detected by TLC, HPLC, LC-MS and NMR analysis.", 
  "author": [
    {
      "family": "Ispirli", 
      "given": " Humeyra"
    }, 
    {
      "family": "Yuzer", 
      "given": " Mustafa Onur"
    }, 
    {
      "family": "Skory", 
      "given": " Christopher"
    }, 
    {
      "family": "Colquhoun", 
      "given": " Ian J."
    }, 
    {
      "family": "Sagdic", 
      "given": " Osman"
    }, 
    {
      "family": "Dertli", 
      "given": " Enes"
    }
  ], 
  "container_title": "BIOCATALYSIS AND BIOTRANSFORMATION", 
  "id": "71257", 
  "issue": "6", 
  "issued": {
    "date-parts": [
      [
        2019, 
        1, 
        1
      ]
    ]
  }, 
  "page": "421-430", 
  "title": "Characterization of a glucansucrase from Lactobacillus reuteri E81 and production of malto-oligosaccharides", 
  "type": "article-journal", 
  "volume": "37"
}