1 Ocak 2019 Dergi makalesi Açık Erişim

Kasnak, Goekhan; Kononen, Eija; Syrjanen, Stina; Gursoy, Mervi; Zeidan-Chulia, Fares; Firatli, Erhan; Gursoy, Ulvi K.

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  <dc:creator>Kasnak, Goekhan</dc:creator>
  <dc:creator>Kononen, Eija</dc:creator>
  <dc:creator>Syrjanen, Stina</dc:creator>
  <dc:creator>Gursoy, Mervi</dc:creator>
  <dc:creator>Zeidan-Chulia, Fares</dc:creator>
  <dc:creator>Firatli, Erhan</dc:creator>
  <dc:creator>Gursoy, Ulvi K.</dc:creator>
  <dc:date>2019-01-01</dc:date>
  <dc:description>ObjectiveBacterial or tobacco-related insults induce oxidative stress in gingival keratinocytes. The aim of this study was to investigate anti-oxidative and cytokine responses of human gingival keratinocytes (HMK cells) against Porphyromonas gingivalis lipopolysaccharide (Pg LPS), nicotine, and 4-nitroquinoline N-oxide (4-NQO).Materials and methodsHMK cells were incubated with Pg LPS (1 mu l/ml), nicotine (1.54mM), and 4-NQO (1 mu M) for 24h. Intracellular and extracellular levels of interleukin (IL)-1, IL-1 receptor antagonist (IL-1Ra), IL-8, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) were measured with the Luminex (R) xMAP technique, and nuclear factor, erythroid 2 like 2 (NFE2L2/NRF2) and 8-oxoguanine DNA glycosylase (OGG1) with Western blots. Data were statistically analyzed by two-way ANOVA with Bonferroni correction.ResultsAll tested oxidative stress inducers increased intracellular OGG1 levels, whereas only nicotine and 4-NQO induced NFE2L2/NRF2 levels. Nicotine, 4-NQO, and their combinational applications with Pg LPS induced the secretions of IL-1 and IL-1Ra, while that of IL-8 was inhibited by the presence of Pg LPS. MCP-1 secretion was suppressed by nicotine, alone and together with Pg LPS, while 4-NQO activated its secretion. Treatment of HMK cells with Pg LPS, nicotine, 4-NQO, or their combinations did not affect VEGF levels.ConclusionPg LPS, nicotine, and 4-NQO induce oxidative stress and regulate anti-oxidative response and cytokine expressions in human gingival keratinocytes differently. These results may indicate that bacterial and tobacco-related insults regulate distinct pathways.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/67721</dc:identifier>
  <dc:identifier>oai:zenodo.org:67721</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>MOLECULAR AND CELLULAR BIOCHEMISTRY 452(1-2) 63-70</dc:source>
  <dc:title>NFE2L2/NRF2, OGG1, and cytokine responses of human gingival keratinocytes against oxidative insults of various origin</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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