1 Ocak 2019 Dergi makalesi Açık Erişim

Kasnak, Goekhan; Kononen, Eija; Syrjanen, Stina; Gursoy, Mervi; Zeidan-Chulia, Fares; Firatli, Erhan; Gursoy, Ulvi K.

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{
  "DOI": "10.1007/s11010-018-3412-y", 
  "abstract": "ObjectiveBacterial or tobacco-related insults induce oxidative stress in gingival keratinocytes. The aim of this study was to investigate anti-oxidative and cytokine responses of human gingival keratinocytes (HMK cells) against Porphyromonas gingivalis lipopolysaccharide (Pg LPS), nicotine, and 4-nitroquinoline N-oxide (4-NQO).Materials and methodsHMK cells were incubated with Pg LPS (1 mu l/ml), nicotine (1.54mM), and 4-NQO (1 mu M) for 24h. Intracellular and extracellular levels of interleukin (IL)-1, IL-1 receptor antagonist (IL-1Ra), IL-8, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) were measured with the Luminex (R) xMAP technique, and nuclear factor, erythroid 2 like 2 (NFE2L2/NRF2) and 8-oxoguanine DNA glycosylase (OGG1) with Western blots. Data were statistically analyzed by two-way ANOVA with Bonferroni correction.ResultsAll tested oxidative stress inducers increased intracellular OGG1 levels, whereas only nicotine and 4-NQO induced NFE2L2/NRF2 levels. Nicotine, 4-NQO, and their combinational applications with Pg LPS induced the secretions of IL-1 and IL-1Ra, while that of IL-8 was inhibited by the presence of Pg LPS. MCP-1 secretion was suppressed by nicotine, alone and together with Pg LPS, while 4-NQO activated its secretion. Treatment of HMK cells with Pg LPS, nicotine, 4-NQO, or their combinations did not affect VEGF levels.ConclusionPg LPS, nicotine, and 4-NQO induce oxidative stress and regulate anti-oxidative response and cytokine expressions in human gingival keratinocytes differently. These results may indicate that bacterial and tobacco-related insults regulate distinct pathways.", 
  "author": [
    {
      "family": "Kasnak", 
      "given": " Goekhan"
    }, 
    {
      "family": "Kononen", 
      "given": " Eija"
    }, 
    {
      "family": "Syrjanen", 
      "given": " Stina"
    }, 
    {
      "family": "Gursoy", 
      "given": " Mervi"
    }, 
    {
      "family": "Zeidan-Chulia", 
      "given": " Fares"
    }, 
    {
      "family": "Firatli", 
      "given": " Erhan"
    }, 
    {
      "family": "Gursoy", 
      "given": " Ulvi K."
    }
  ], 
  "container_title": "MOLECULAR AND CELLULAR BIOCHEMISTRY", 
  "id": "67721", 
  "issue": "1-2", 
  "issued": {
    "date-parts": [
      [
        2019, 
        1, 
        1
      ]
    ]
  }, 
  "page": "63-70", 
  "title": "NFE2L2/NRF2, OGG1, and cytokine responses of human gingival keratinocytes against oxidative insults of various origin", 
  "type": "article-journal", 
  "volume": "452"
}