1 Ocak 2019 Dergi makalesi Açık Erişim

Kasnak, Goekhan; Kononen, Eija; Syrjanen, Stina; Gursoy, Mervi; Zeidan-Chulia, Fares; Firatli, Erhan; Gursoy, Ulvi K.

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/67721</identifier>
  <creators>
    <creator>
      <creatorName>Kasnak, Goekhan</creatorName>
      <givenName>Goekhan</givenName>
      <familyName>Kasnak</familyName>
    </creator>
    <creator>
      <creatorName>Kononen, Eija</creatorName>
      <givenName>Eija</givenName>
      <familyName>Kononen</familyName>
    </creator>
    <creator>
      <creatorName>Syrjanen, Stina</creatorName>
      <givenName>Stina</givenName>
      <familyName>Syrjanen</familyName>
    </creator>
    <creator>
      <creatorName>Gursoy, Mervi</creatorName>
      <givenName>Mervi</givenName>
      <familyName>Gursoy</familyName>
      <affiliation>Univ Turku, Inst Dent, Lemminkaisenkatu 2, FIN-20520 Turku, Finland</affiliation>
    </creator>
    <creator>
      <creatorName>Zeidan-Chulia, Fares</creatorName>
      <givenName>Fares</givenName>
      <familyName>Zeidan-Chulia</familyName>
    </creator>
    <creator>
      <creatorName>Firatli, Erhan</creatorName>
      <givenName>Erhan</givenName>
      <familyName>Firatli</familyName>
      <affiliation>Istanbul Univ, Fac Dent, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Gursoy, Ulvi K.</creatorName>
      <givenName>Ulvi K.</givenName>
      <familyName>Gursoy</familyName>
      <affiliation>Univ Turku, Inst Dent, Lemminkaisenkatu 2, FIN-20520 Turku, Finland</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Nfe2L2/Nrf2, Ogg1, And Cytokine Responses Of Human Gingival Keratinocytes Against Oxidative Insults Of Various Origin</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2019</publicationYear>
  <dates>
    <date dateType="Issued">2019-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/67721</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1007/s11010-018-3412-y</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">ObjectiveBacterial or tobacco-related insults induce oxidative stress in gingival keratinocytes. The aim of this study was to investigate anti-oxidative and cytokine responses of human gingival keratinocytes (HMK cells) against Porphyromonas gingivalis lipopolysaccharide (Pg LPS), nicotine, and 4-nitroquinoline N-oxide (4-NQO).Materials and methodsHMK cells were incubated with Pg LPS (1 mu l/ml), nicotine (1.54mM), and 4-NQO (1 mu M) for 24h. Intracellular and extracellular levels of interleukin (IL)-1, IL-1 receptor antagonist (IL-1Ra), IL-8, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) were measured with the Luminex (R) xMAP technique, and nuclear factor, erythroid 2 like 2 (NFE2L2/NRF2) and 8-oxoguanine DNA glycosylase (OGG1) with Western blots. Data were statistically analyzed by two-way ANOVA with Bonferroni correction.ResultsAll tested oxidative stress inducers increased intracellular OGG1 levels, whereas only nicotine and 4-NQO induced NFE2L2/NRF2 levels. Nicotine, 4-NQO, and their combinational applications with Pg LPS induced the secretions of IL-1 and IL-1Ra, while that of IL-8 was inhibited by the presence of Pg LPS. MCP-1 secretion was suppressed by nicotine, alone and together with Pg LPS, while 4-NQO activated its secretion. Treatment of HMK cells with Pg LPS, nicotine, 4-NQO, or their combinations did not affect VEGF levels.ConclusionPg LPS, nicotine, and 4-NQO induce oxidative stress and regulate anti-oxidative response and cytokine expressions in human gingival keratinocytes differently. These results may indicate that bacterial and tobacco-related insults regulate distinct pathways.</description>
  </descriptions>
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