1 Ocak 2019 Dergi makalesi Açık Erişim

Kasnak, Goekhan; Kononen, Eija; Syrjanen, Stina; Gursoy, Mervi; Zeidan-Chulia, Fares; Firatli, Erhan; Gursoy, Ulvi K.

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    "creators": [
      {
        "name": "Kasnak, Goekhan"
      }, 
      {
        "name": "Kononen, Eija"
      }, 
      {
        "name": "Syrjanen, Stina"
      }, 
      {
        "affiliation": "Univ Turku, Inst Dent, Lemminkaisenkatu 2, FIN-20520 Turku, Finland", 
        "name": "Gursoy, Mervi"
      }, 
      {
        "name": "Zeidan-Chulia, Fares"
      }, 
      {
        "affiliation": "Istanbul Univ, Fac Dent, Istanbul, Turkey", 
        "name": "Firatli, Erhan"
      }, 
      {
        "affiliation": "Univ Turku, Inst Dent, Lemminkaisenkatu 2, FIN-20520 Turku, Finland", 
        "name": "Gursoy, Ulvi K."
      }
    ], 
    "description": "ObjectiveBacterial or tobacco-related insults induce oxidative stress in gingival keratinocytes. The aim of this study was to investigate anti-oxidative and cytokine responses of human gingival keratinocytes (HMK cells) against Porphyromonas gingivalis lipopolysaccharide (Pg LPS), nicotine, and 4-nitroquinoline N-oxide (4-NQO).Materials and methodsHMK cells were incubated with Pg LPS (1 mu l/ml), nicotine (1.54mM), and 4-NQO (1 mu M) for 24h. Intracellular and extracellular levels of interleukin (IL)-1, IL-1 receptor antagonist (IL-1Ra), IL-8, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) were measured with the Luminex (R) xMAP technique, and nuclear factor, erythroid 2 like 2 (NFE2L2/NRF2) and 8-oxoguanine DNA glycosylase (OGG1) with Western blots. Data were statistically analyzed by two-way ANOVA with Bonferroni correction.ResultsAll tested oxidative stress inducers increased intracellular OGG1 levels, whereas only nicotine and 4-NQO induced NFE2L2/NRF2 levels. Nicotine, 4-NQO, and their combinational applications with Pg LPS induced the secretions of IL-1 and IL-1Ra, while that of IL-8 was inhibited by the presence of Pg LPS. MCP-1 secretion was suppressed by nicotine, alone and together with Pg LPS, while 4-NQO activated its secretion. Treatment of HMK cells with Pg LPS, nicotine, 4-NQO, or their combinations did not affect VEGF levels.ConclusionPg LPS, nicotine, and 4-NQO induce oxidative stress and regulate anti-oxidative response and cytokine expressions in human gingival keratinocytes differently. These results may indicate that bacterial and tobacco-related insults regulate distinct pathways.", 
    "doi": "10.1007/s11010-018-3412-y", 
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    "journal": {
      "issue": "1-2", 
      "pages": "63-70", 
      "title": "MOLECULAR AND CELLULAR BIOCHEMISTRY", 
      "volume": "452"
    }, 
    "license": {
      "id": "cc-by"
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    "publication_date": "2019-01-01", 
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    "resource_type": {
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      "title": "Dergi makalesi", 
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    "title": "NFE2L2/NRF2, OGG1, and cytokine responses of human gingival keratinocytes against oxidative insults of various origin"
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