1 Ocak 2019 Dergi makalesi Açık Erişim

Kasnak, Goekhan; Kononen, Eija; Syrjanen, Stina; Gursoy, Mervi; Zeidan-Chulia, Fares; Firatli, Erhan; Gursoy, Ulvi K.

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{
  "@context": "https://schema.org/", 
  "@id": 67721, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Kasnak, Goekhan"
    }, 
    {
      "@type": "Person", 
      "name": "Kononen, Eija"
    }, 
    {
      "@type": "Person", 
      "name": "Syrjanen, Stina"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Univ Turku, Inst Dent, Lemminkaisenkatu 2, FIN-20520 Turku, Finland", 
      "name": "Gursoy, Mervi"
    }, 
    {
      "@type": "Person", 
      "name": "Zeidan-Chulia, Fares"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Istanbul Univ, Fac Dent, Istanbul, Turkey", 
      "name": "Firatli, Erhan"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Univ Turku, Inst Dent, Lemminkaisenkatu 2, FIN-20520 Turku, Finland", 
      "name": "Gursoy, Ulvi K."
    }
  ], 
  "datePublished": "2019-01-01", 
  "description": "ObjectiveBacterial or tobacco-related insults induce oxidative stress in gingival keratinocytes. The aim of this study was to investigate anti-oxidative and cytokine responses of human gingival keratinocytes (HMK cells) against Porphyromonas gingivalis lipopolysaccharide (Pg LPS), nicotine, and 4-nitroquinoline N-oxide (4-NQO).Materials and methodsHMK cells were incubated with Pg LPS (1 mu l/ml), nicotine (1.54mM), and 4-NQO (1 mu M) for 24h. Intracellular and extracellular levels of interleukin (IL)-1, IL-1 receptor antagonist (IL-1Ra), IL-8, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) were measured with the Luminex (R) xMAP technique, and nuclear factor, erythroid 2 like 2 (NFE2L2/NRF2) and 8-oxoguanine DNA glycosylase (OGG1) with Western blots. Data were statistically analyzed by two-way ANOVA with Bonferroni correction.ResultsAll tested oxidative stress inducers increased intracellular OGG1 levels, whereas only nicotine and 4-NQO induced NFE2L2/NRF2 levels. Nicotine, 4-NQO, and their combinational applications with Pg LPS induced the secretions of IL-1 and IL-1Ra, while that of IL-8 was inhibited by the presence of Pg LPS. MCP-1 secretion was suppressed by nicotine, alone and together with Pg LPS, while 4-NQO activated its secretion. Treatment of HMK cells with Pg LPS, nicotine, 4-NQO, or their combinations did not affect VEGF levels.ConclusionPg LPS, nicotine, and 4-NQO induce oxidative stress and regulate anti-oxidative response and cytokine expressions in human gingival keratinocytes differently. These results may indicate that bacterial and tobacco-related insults regulate distinct pathways.", 
  "headline": "NFE2L2/NRF2, OGG1, and cytokine responses of human gingival keratinocytes against oxidative insults of various origin", 
  "identifier": 67721, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "NFE2L2/NRF2, OGG1, and cytokine responses of human gingival keratinocytes against oxidative insults of various origin", 
  "url": "https://aperta.ulakbim.gov.tr/record/67721"
}