1 Ocak 2017 Dergi makalesi Açık Erişim

Sahin, A.; Spiroux, F.; Guedon, I.; Arslan, F. B.; Sarcan, E. T.; Ozkan, T.; Colak, N.; Yuksel, S.; Ozdemir, S.; Ozdemir, B.; Akbas, S.; Ultav, G.; Aktas, Y.; Capan, Y.

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{
  "DOI": "10.1691/ph.2017.7015", 
  "abstract": "In the preparation of nanoparticles (NPs) by the nanoprecipitation method, emulsifiers play a key role for NPs' characteristics. The present study aimed to investigate the combined emulsifier effect on ibuprofen loaded poly( lactic-co-glycolic acid) (PLGA) NPs' characteristics and anticancer activity. Ibuprofen loaded PLGA NPs were prepared by nanoprecipitation using different concentrations of PVA (poly(vinyl alcohol)) or PVA TPGS (d-alpha-tocopherol polyethylene glycol 1000 succinate) combination as emulsifier. It was found that encapsulation efficiencies of NPs varied between 17.9 and 41.9 % and the highest encapsulation efficiency was obtained with 0.5% PVA + 0.1% TPGS (coded as PLGA PVA/TPGS NPs). PLGA PVA/TPGS NPs were characterized and compared with PLGA PVA NPs, which was obtained by 0.5% PVA alone. Polydispersity index of PLGA PVA/TPGS and PLGA PVA NPs were found to be 0.08 and 0.15, respectively. Incorporation of TPGS with PVA slightly decreased the initial ibuprofen release. Transmission electron microscopy analyses demonstrated a nearly uniform particle size distribution and spherical particle shape of the PLGA PVA/TPGS NPs. Additionally, PLGA PVA/TPGS NPs were significantly more cytotoxic than PLGA PVA NPs on the MCF-7 (human breast adenocarcinoma cells) and Caco-2 (human epithelial colorectal adenocarcinoma) cells (p<0.05). Also PLGA PVA/TPGS NPs were not cytotoxic on normal cells (L929, mouse healthy fibroblast cells) (p>0.05). In conclusion, these results indicated that using a combination of TPGS and PVA as an emulsifier in nanoprecipitation could be a promising approach for preparing ibuprofen loaded PLGA NPs because of their improved characteristics and anticancer activity.", 
  "author": [
    {
      "family": "Sahin", 
      "given": " A."
    }, 
    {
      "family": "Spiroux", 
      "given": " F."
    }, 
    {
      "family": "Guedon", 
      "given": " I."
    }, 
    {
      "family": "Arslan", 
      "given": " F. B."
    }, 
    {
      "family": "Sarcan", 
      "given": " E. T."
    }, 
    {
      "family": "Ozkan", 
      "given": " T."
    }, 
    {
      "family": "Colak", 
      "given": " N."
    }, 
    {
      "family": "Yuksel", 
      "given": " S."
    }, 
    {
      "family": "Ozdemir", 
      "given": " S."
    }, 
    {
      "family": "Ozdemir", 
      "given": " B."
    }, 
    {
      "family": "Akbas", 
      "given": " S."
    }, 
    {
      "family": "Ultav", 
      "given": " G."
    }, 
    {
      "family": "Aktas", 
      "given": " Y."
    }, 
    {
      "family": "Capan", 
      "given": " Y."
    }
  ], 
  "container_title": "PHARMAZIE", 
  "id": "47803", 
  "issue": "9", 
  "issued": {
    "date-parts": [
      [
        2017, 
        1, 
        1
      ]
    ]
  }, 
  "page": "525-528", 
  "title": "Using PVA and TPGS as combined emulsifier in nanoprecipitation method improves characteristics and anticancer activity of ibuprofen loaded PLGA nanoparticles", 
  "type": "article-journal", 
  "volume": "72"
}