1 Ocak 2018 Dergi makalesi Açık Erişim

Armutcu, Canan; Uzun, Lokman; Denizli, Adil

Citation Style Language JSON

{
  "DOI": "10.1016/j.chroma.2018.07.057", 
  "abstract": "Automated on-line two-dimensional high-performance liquid chromatography method (2D-HPLC) is proposed to determine Ochratoxin A (OTA) in food samples as an alternative to OTA immunoaffinity column (IAC). An on-line 2D-HPLC system is designed for the analysis of OTA using an affinity-based monolithic column in the first dimension and reversed-phase C18 column in the second dimension. Initially, optimal OTA separation efficiency is determined through traditional HPLC system consisting of a P(HEMAPA) monolithic column coupled with HPLC system. Secondly, after providing optimum conditions, OTA determination was investigated through the 2D-HPLC system. According to results, 2D-HPLC system showed good linearity in the range 0.5 to 20 ng/mL with limit of detection (LOD) and limit of quantification (LOQ) values of 21.2 pg/mL and 64.3 pg/mL, respectively. The P(HEMAPA)-4 monolithic column displayed good recovery of OTA ranging from 104.34% to 107.33%. Relative standard deviations (RSD) varied in the range 0.21% to 1.31% thus indicating the efficiency of P(HEMAPA)-4 monolithic column developed for OTA. (C) 2018 Elsevier B.V. All rights reserved.", 
  "author": [
    {
      "family": "Armutcu", 
      "given": " Canan"
    }, 
    {
      "family": "Uzun", 
      "given": " Lokman"
    }, 
    {
      "family": "Denizli", 
      "given": " Adil"
    }
  ], 
  "container_title": "JOURNAL OF CHROMATOGRAPHY A", 
  "id": "33061", 
  "issued": {
    "date-parts": [
      [
        2018, 
        1, 
        1
      ]
    ]
  }, 
  "page": "139-148", 
  "title": "Determination of Ochratoxin A traces in foodstuffs: Comparison of an automated on-line two-dimensional high-performance liquid chromatography and off-line immunoaffinity-high-performance liquid chromatography system", 
  "type": "article-journal", 
  "volume": "1569"
}