1 Ocak 2010 Dergi makalesi Açık Erişim

Eyigor, Ozhan; Minbay, Zehra; Cavusoglu, Ilkin

Citation Style Language JSON

{
  "DOI": "10.1007/s12020-009-9284-x", 
  "abstract": "Orexin neuropeptides participate in the regulation of feeding as well as the regulation and maintenance of wakefulness and the cognitive functions. Orexin A and B share a common precursor, prepro-orexin and neurons are localized in the lateral hypothalamus. Physiological studies showed that these neurons are regulated by glutamatergic innervations. We aimed to assess the effects of kainic acid as a potent agonist for non-NMDA glutamate receptors in the activation of orexin neurons. We also analyzed the effect of glutamate antagonist CNQX, injected prior to kainic acid, on this activation. Expression of c-Fos protein was used as a marker for neuronal activation. Dual immunohistochemical labeling was performed for prepro-orexin and c-Fos and the percentages of c-Fos-expressing orexin neurons were obtained for control, kainic acid, and CNQX groups. Kainic acid injection caused statistically significant increase in the number of c-Fos-positive neurons when compared to control group (62.69 and 36.31%, respectively). Activation of orexin neurons was blocked, in part, by CNQX (43.36%). In the light of these results, it is concluded that glutamate takes part in the regulation of orexin neurons and partially exerts its effects through non-NMDA glutamate receptors and that orexin neurons express functional non-NMDA receptors.", 
  "author": [
    {
      "family": "Eyigor", 
      "given": " Ozhan"
    }, 
    {
      "family": "Minbay", 
      "given": " Zehra"
    }, 
    {
      "family": "Cavusoglu", 
      "given": " Ilkin"
    }
  ], 
  "container_title": "ENDOCRINE", 
  "id": "28437", 
  "issue": "1", 
  "issued": {
    "date-parts": [
      [
        2010, 
        1, 
        1
      ]
    ]
  }, 
  "page": "167-172", 
  "title": "Activation of orexin neurons through non-NMDA glutamate receptors evidenced by c-Fos immunohistochemistry", 
  "type": "article-journal", 
  "volume": "37"
}