Dergi makalesi Açık Erişim
Eyigor, Ozhan; Minbay, Zehra; Cavusoglu, Ilkin
{ "DOI": "10.1007/s12020-009-9284-x", "abstract": "Orexin neuropeptides participate in the regulation of feeding as well as the regulation and maintenance of wakefulness and the cognitive functions. Orexin A and B share a common precursor, prepro-orexin and neurons are localized in the lateral hypothalamus. Physiological studies showed that these neurons are regulated by glutamatergic innervations. We aimed to assess the effects of kainic acid as a potent agonist for non-NMDA glutamate receptors in the activation of orexin neurons. We also analyzed the effect of glutamate antagonist CNQX, injected prior to kainic acid, on this activation. Expression of c-Fos protein was used as a marker for neuronal activation. Dual immunohistochemical labeling was performed for prepro-orexin and c-Fos and the percentages of c-Fos-expressing orexin neurons were obtained for control, kainic acid, and CNQX groups. Kainic acid injection caused statistically significant increase in the number of c-Fos-positive neurons when compared to control group (62.69 and 36.31%, respectively). Activation of orexin neurons was blocked, in part, by CNQX (43.36%). In the light of these results, it is concluded that glutamate takes part in the regulation of orexin neurons and partially exerts its effects through non-NMDA glutamate receptors and that orexin neurons express functional non-NMDA receptors.", "author": [ { "family": "Eyigor", "given": " Ozhan" }, { "family": "Minbay", "given": " Zehra" }, { "family": "Cavusoglu", "given": " Ilkin" } ], "container_title": "ENDOCRINE", "id": "28437", "issue": "1", "issued": { "date-parts": [ [ 2010, 1, 1 ] ] }, "page": "167-172", "title": "Activation of orexin neurons through non-NMDA glutamate receptors evidenced by c-Fos immunohistochemistry", "type": "article-journal", "volume": "37" }
Görüntülenme | 25 |
İndirme | 8 |
Veri hacmi | 1.4 kB |
Tekil görüntülenme | 24 |
Tekil indirme | 8 |