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Chemoenzymatic Conversion of Linoleic Acid into Conjugated Linoleic Acid

Demir, Ayhan S.; Talpur, Farah N.


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        "affiliation": "Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey", 
        "name": "Demir, Ayhan S."
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        "name": "Talpur, Farah N."
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    "description": "An efficient chemoenzymatic method for preparing conjugated linoleic acid (CLA) using free linoleic acid (LA) as a substrate is described. In the first step, LA was transformed into 10-hydroxy-cis-12-octadecenoic acid (HA) by the whole cells of Lactobacillus plantarum after 48 h of incubation. The preincubation of whole cells with 0.03% LA resulted in a better yield of HA (480 mg/g) compared to cells grown without LA. In a second fast microwave step, HA was converted to cis-9, trans-11-octadecadienoic acid in the presence of iodine as a catalyst over a silica gel surface. The advantage of this method in preparing cis-9,trans-11 CLA is simple via the whole cell bioconversion of LA into HA via L. plantarum followed by the fast microwave-assisted synthesis of cis-9,trans-11 CLA in higher yields.", 
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      "issue": "3", 
      "pages": "1646-1652", 
      "title": "JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY", 
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    "title": "Chemoenzymatic Conversion of Linoleic Acid into Conjugated Linoleic Acid"
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