1 Ocak 2021 Dergi makalesi Açık Erişim

Ates, Mehmet Burak; Ortatatli, Mustafa

MARC21 XML

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2021-01-01</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">Selcuk Univ, Fac Vet Med, Dept Pathol, TR-42130 Konya, Turkey</subfield>
    <subfield code="a">Ates, Mehmet Burak</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield>
    <subfield code="a">Creative Commons Attribution</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Selcuk Univ, Fac Vet Med, Dept Pathol, TR-42130 Konya, Turkey</subfield>
    <subfield code="a">Ortatatli, Mustafa</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="o">oai:aperta.ulakbim.gov.tr:234742</subfield>
    <subfield code="p">user-tubitak-destekli-proje-yayinlari</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">The primary pathway of the detoxification of aflatoxin (AF) metabolites occurring at the end of phase-1 biotransformation is glutathione (GSH) conjugation via glutathione-S-transferase (GST) enzyme in phase-2. In this study, the activity of Nigella sativa seeds (NS) and thymoquinone (TQ) on phase-2 detoxification pathways of AF was investigated in light of GSH, GST alpha-3 (GSTA3), and AFB(1)-DNA adducts detected by the immunohistochemical method in broilers' livers. One-hundred twenty chickens at one-day-old were divided into six groups as the control (CNT), AF, TQ, NS, AF + TQ, and AF + NS groups and fed for 28 days. AF, TQ, and NS were added to the relevant diets at 2 mg/kg, 300 mg/kg, and 5%, respectively. The administration of AF alone strongly stimulated the formation of AFB(1)-DNA adduct and reduced GSH and GSTA3 levels in hepatocytes (p &amp;lt; 0,01). In contrast, TQ and NS were found to reduce significantly the amount of AFB(1)-DNA adducts in AF + TQ and AF + NS groups (p &amp;lt; 0,01). We concluded that NS and TQ achieved this by increasing GSH and GSTA3 levels (p &amp;lt; 0,01) thanks to their antioxidant properties, and hence detoxifying the reactive metabolites of AF. Also, we consider that the AFB(1)-DNA adduct constituted in 28 days can be used as a biomarker for exposure to AF in broilers.</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.1016/j.toxicon.2021.01.020</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <controlfield tag="005">20221007090657.0</controlfield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">The effects of Nigella sativa seeds and thymoquinone on aflatoxin phase-2 detoxification through glutathione and glutathione-S-transferase alpha-3, and the relationship between aflatoxin B-1-DNA adducts in broilers</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="c">86-92</subfield>
    <subfield code="p">TOXICON</subfield>
    <subfield code="v">193</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="2">opendefinition.org</subfield>
    <subfield code="a">cc-by</subfield>
  </datafield>
  <controlfield tag="001">234742</controlfield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-tubitak-destekli-proje-yayinlari</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="b">article</subfield>
    <subfield code="a">publication</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="u">https://aperta.ulakbim.gov.trrecord/234742/files/bib-22a38b3e-bf1d-4e6e-9f49-703f0f34131f.txt</subfield>
    <subfield code="z">md5:3c5928ac146502328e09539ba1938ba3</subfield>
    <subfield code="s">275</subfield>
  </datafield>
</record>