1 Ocak 2021 Dergi makalesi Açık Erişim

Ates, Mehmet Burak; Ortatatli, Mustafa

Dublin Core

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  <dc:creator>Ates, Mehmet Burak</dc:creator>
  <dc:creator>Ortatatli, Mustafa</dc:creator>
  <dc:date>2021-01-01</dc:date>
  <dc:description>The primary pathway of the detoxification of aflatoxin (AF) metabolites occurring at the end of phase-1 biotransformation is glutathione (GSH) conjugation via glutathione-S-transferase (GST) enzyme in phase-2. In this study, the activity of Nigella sativa seeds (NS) and thymoquinone (TQ) on phase-2 detoxification pathways of AF was investigated in light of GSH, GST alpha-3 (GSTA3), and AFB(1)-DNA adducts detected by the immunohistochemical method in broilers' livers. One-hundred twenty chickens at one-day-old were divided into six groups as the control (CNT), AF, TQ, NS, AF + TQ, and AF + NS groups and fed for 28 days. AF, TQ, and NS were added to the relevant diets at 2 mg/kg, 300 mg/kg, and 5%, respectively. The administration of AF alone strongly stimulated the formation of AFB(1)-DNA adduct and reduced GSH and GSTA3 levels in hepatocytes (p &lt; 0,01). In contrast, TQ and NS were found to reduce significantly the amount of AFB(1)-DNA adducts in AF + TQ and AF + NS groups (p &lt; 0,01). We concluded that NS and TQ achieved this by increasing GSH and GSTA3 levels (p &lt; 0,01) thanks to their antioxidant properties, and hence detoxifying the reactive metabolites of AF. Also, we consider that the AFB(1)-DNA adduct constituted in 28 days can be used as a biomarker for exposure to AF in broilers.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/234742</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:234742</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>TOXICON 193 86-92</dc:source>
  <dc:title>The effects of Nigella sativa seeds and thymoquinone on aflatoxin phase-2 detoxification through glutathione and glutathione-S-transferase alpha-3, and the relationship between aflatoxin B-1-DNA adducts in broilers</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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