1 Ocak 2021 Dergi makalesi Açık Erişim

Ates, Mehmet Burak; Ortatatli, Mustafa

Citation Style Language JSON

{
  "DOI": "10.1016/j.toxicon.2021.01.020", 
  "abstract": "The primary pathway of the detoxification of aflatoxin (AF) metabolites occurring at the end of phase-1 biotransformation is glutathione (GSH) conjugation via glutathione-S-transferase (GST) enzyme in phase-2. In this study, the activity of Nigella sativa seeds (NS) and thymoquinone (TQ) on phase-2 detoxification pathways of AF was investigated in light of GSH, GST alpha-3 (GSTA3), and AFB(1)-DNA adducts detected by the immunohistochemical method in broilers' livers. One-hundred twenty chickens at one-day-old were divided into six groups as the control (CNT), AF, TQ, NS, AF + TQ, and AF + NS groups and fed for 28 days. AF, TQ, and NS were added to the relevant diets at 2 mg/kg, 300 mg/kg, and 5%, respectively. The administration of AF alone strongly stimulated the formation of AFB(1)-DNA adduct and reduced GSH and GSTA3 levels in hepatocytes (p < 0,01). In contrast, TQ and NS were found to reduce significantly the amount of AFB(1)-DNA adducts in AF + TQ and AF + NS groups (p < 0,01). We concluded that NS and TQ achieved this by increasing GSH and GSTA3 levels (p < 0,01) thanks to their antioxidant properties, and hence detoxifying the reactive metabolites of AF. Also, we consider that the AFB(1)-DNA adduct constituted in 28 days can be used as a biomarker for exposure to AF in broilers.", 
  "author": [
    {
      "family": "Ates", 
      "given": " Mehmet Burak"
    }, 
    {
      "family": "Ortatatli", 
      "given": " Mustafa"
    }
  ], 
  "container_title": "TOXICON", 
  "id": "234742", 
  "issued": {
    "date-parts": [
      [
        2021, 
        1, 
        1
      ]
    ]
  }, 
  "page": "86-92", 
  "title": "The effects of Nigella sativa seeds and thymoquinone on aflatoxin phase-2 detoxification through glutathione and glutathione-S-transferase alpha-3, and the relationship between aflatoxin B-1-DNA adducts in broilers", 
  "type": "article-journal", 
  "volume": "193"
}