1 Ocak 2021 Dergi makalesi Açık Erişim

Ates, Mehmet Burak; Ortatatli, Mustafa

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/234742</identifier>
  <creators>
    <creator>
      <creatorName>Ates, Mehmet Burak</creatorName>
      <givenName>Mehmet Burak</givenName>
      <familyName>Ates</familyName>
      <affiliation>Selcuk Univ, Fac Vet Med, Dept Pathol, TR-42130 Konya, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ortatatli, Mustafa</creatorName>
      <givenName>Mustafa</givenName>
      <familyName>Ortatatli</familyName>
      <affiliation>Selcuk Univ, Fac Vet Med, Dept Pathol, TR-42130 Konya, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>The Effects Of Nigella Sativa Seeds And Thymoquinone On Aflatoxin Phase-2 Detoxification Through Glutathione And Glutathione-S-Transferase Alpha-3, And The Relationship Between Aflatoxin B-1-Dna Adducts In Broilers</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/234742</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.toxicon.2021.01.020</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
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  <descriptions>
    <description descriptionType="Abstract">The primary pathway of the detoxification of aflatoxin (AF) metabolites occurring at the end of phase-1 biotransformation is glutathione (GSH) conjugation via glutathione-S-transferase (GST) enzyme in phase-2. In this study, the activity of Nigella sativa seeds (NS) and thymoquinone (TQ) on phase-2 detoxification pathways of AF was investigated in light of GSH, GST alpha-3 (GSTA3), and AFB(1)-DNA adducts detected by the immunohistochemical method in broilers' livers. One-hundred twenty chickens at one-day-old were divided into six groups as the control (CNT), AF, TQ, NS, AF + TQ, and AF + NS groups and fed for 28 days. AF, TQ, and NS were added to the relevant diets at 2 mg/kg, 300 mg/kg, and 5%, respectively. The administration of AF alone strongly stimulated the formation of AFB(1)-DNA adduct and reduced GSH and GSTA3 levels in hepatocytes (p &amp;lt; 0,01). In contrast, TQ and NS were found to reduce significantly the amount of AFB(1)-DNA adducts in AF + TQ and AF + NS groups (p &amp;lt; 0,01). We concluded that NS and TQ achieved this by increasing GSH and GSTA3 levels (p &amp;lt; 0,01) thanks to their antioxidant properties, and hence detoxifying the reactive metabolites of AF. Also, we consider that the AFB(1)-DNA adduct constituted in 28 days can be used as a biomarker for exposure to AF in broilers.</description>
  </descriptions>
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