1 Ocak 2017 Dergi makalesi Açık Erişim

Kuete, V.; Mafodong, F. L. Dongmo; Celik, I.; Fobofou, S. A. T.; Ndontsa, B. L.; Karaosmanoglu, O.; Weissjohann, L. A.; Tane, P.; Koparal, A. T.; Sivas, H.

Citation Style Language JSON

{
  "DOI": "10.1016/j.sajb.2017.03.031", 
  "abstract": "Malignancies constitute a global health concern and chemotherapy remains the main mode of treatment. The present study was designed to evaluate the cytotoxicity of 8 compounds from Desbordesia glaucescens namely lanosta-7,24-dien-3-one (1), friedelanone (2), friedelanol (3), 3,3'-di-O-methylellagic acid (4), 3,3',4'-tri-0-methylellagic acid (5), ellagic acid (6), 3',4'-di-0-methylellagic acid 4-0-beta-o-glucopyranoside (7) and 3,3'-di-0-methylellagic acid 4'-0-beta-c-xylopyranoside (8) against 4 human carcinoma cell lines and normal CRL2120 fibroblasts. The neutral red uptake (NRU) assay was used for cytotoxicity testing. Caspase-Glo assay, cell cycle analysis, measurements of mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were used to evaluate apoptosis induction. Compounds 4 and 6 as well as doxorubicin had IC50 values below 45 pM in the four tested cancer cell lines meanwhile other compounds displayed selective activity. The IC50 values ranged from 11.23 mu M (towards breast adenocarcinoma MCF-7 cells) to 44.65 mu M (colon carcinoma Caco-2 cells) for 4, from 14.07 mu M (towards MCF-7 cells) to 77.73 mu M (Caco-2 cells) for 6 and from 0.07 mu M (towards SPC212 cells) to 1.01 mu M (A549 cells) for doxorubicin. Compound 4 induced apoptosis in MCF-7 cells mediated by MMP loss. The constituents of Desbordesia glaucescens and especially ellagic acid (6) and its derivative 4 are potential cytotoxic compounds that deserve more investigations towards developing novel antiproliferative drugs against human carcinoma. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved.", 
  "author": [
    {
      "family": "Kuete", 
      "given": " V."
    }, 
    {
      "family": "Mafodong", 
      "given": " F. L. Dongmo"
    }, 
    {
      "family": "Celik", 
      "given": " I."
    }, 
    {
      "family": "Fobofou", 
      "given": " S. A. T."
    }, 
    {
      "family": "Ndontsa", 
      "given": " B. L."
    }, 
    {
      "family": "Karaosmanoglu", 
      "given": " O."
    }, 
    {
      "family": "Weissjohann", 
      "given": " L. A."
    }, 
    {
      "family": "Tane", 
      "given": " P."
    }, 
    {
      "family": "Koparal", 
      "given": " A. T."
    }, 
    {
      "family": "Sivas", 
      "given": " H."
    }
  ], 
  "container_title": "SOUTH AFRICAN JOURNAL OF BOTANY", 
  "id": "111174", 
  "issued": {
    "date-parts": [
      [
        2017, 
        1, 
        1
      ]
    ]
  }, 
  "page": "37-43", 
  "title": "In vitro cytotoxicity of compounds isolated from Desbordesia glaucescens against human carcinoma cell lines", 
  "type": "article-journal", 
  "volume": "111"
}