Dergi makalesi Açık Erişim
Bagis, H; Mercan, HO; Kumtepe, Y
<?xml version='1.0' encoding='UTF-8'?> <record xmlns="http://www.loc.gov/MARC21/slim"> <leader>00000nam##2200000uu#4500</leader> <datafield tag="245" ind1=" " ind2=" "> <subfield code="a">Effect of three different cryoprotectant solutions in solid surface vitrification (SSV) techniques on the development rate of vitrified pronuclear-stage mouse embryos</subfield> </datafield> <datafield tag="909" ind1="C" ind2="4"> <subfield code="p">TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES</subfield> <subfield code="v">29</subfield> <subfield code="n">3</subfield> <subfield code="c">621-627</subfield> </datafield> <controlfield tag="001">93971</controlfield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="a">user-tubitak-adresli-yayinlar</subfield> </datafield> <datafield tag="520" ind1=" " ind2=" "> <subfield code="a">The objective of this study was to compare the effects of three different vitrification solutions on the development of pronuclear-stage (PN) Dinnyes mouse embryos into blastocyst stage after vitrification by solid surface vitrification (SSV) technique. For this aim, it was compared three experimental groups and a control group. Experimental groups were distinguished each other by the vitrification solution used in SSV. It was used 4% Ethylene Glycol (EG) at 37 degrees C equilibration temperature in the first group (SSV-EG), 4% Dimethyl Sulfoxide (DMSO) at room temperature in the second group (SSV-DMSO) and 4% Propylene Glycol (PG) at room temperature in the third group (SSV-PG). After vitrification, the survived embryos were cultured to blastocyst stage in KSOM. It was determined a significant difference between the groups of SSV-EG and SSV-PG at developing rate to 2-cell stage (P &lt; 0.05). Similarly, SSV-PG demonstrated significant differences with SSV-DMSO and the control group at developing rate to 3-8-cell stage (P &lt; 0.05). When compared the rates of developing to morula stage among the groups, it was determined significant differences between SSV-PG and the control group at (P &lt; 0.05); and between SSV-DMSO and SSV-PG (P &lt; 0.01). Finally, it was compared the developing rates into blastocyst stage and found that SSV-EG demonstrated significant differences with SSV-PG and SSV-DMSO (P &lt; 0.01). This study has shown that EG, DMSO and PG with trehalose can be used effectively as a cryoprotective agent in the quick freezing of pronuclear-stage mouse embryos. Finally, additional studies are needed to optimize the SSV method in further stage mouse embryos.</subfield> </datafield> <datafield tag="650" ind1="1" ind2="7"> <subfield code="2">opendefinition.org</subfield> <subfield code="a">cc-by</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Mercan, HO</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Kumtepe, Y</subfield> </datafield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="b">article</subfield> <subfield code="a">publication</subfield> </datafield> <datafield tag="542" ind1=" " ind2=" "> <subfield code="l">open</subfield> </datafield> <datafield tag="100" ind1=" " ind2=" "> <subfield code="a">Bagis, H</subfield> </datafield> <datafield tag="260" ind1=" " ind2=" "> <subfield code="c">2005-01-01</subfield> </datafield> <controlfield tag="005">20210316125556.0</controlfield> <datafield tag="773" ind1=" " ind2=" "> <subfield code="n">doi</subfield> <subfield code="a">10.81043/aperta.93970</subfield> <subfield code="i">isVersionOf</subfield> </datafield> <datafield tag="909" ind1="C" ind2="O"> <subfield code="o">oai:zenodo.org:93971</subfield> <subfield code="p">user-tubitak-adresli-yayinlar</subfield> </datafield> <datafield tag="856" ind1="4" ind2=" "> <subfield code="z">md5:9a7da9aa8f4d416219202f7e5b7aa85a</subfield> <subfield code="s">276</subfield> <subfield code="u">https://aperta.ulakbim.gov.trrecord/93971/files/bib-35255bf0-dc1d-4762-828b-4e099c29145b.txt</subfield> </datafield> <datafield tag="540" ind1=" " ind2=" "> <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield> <subfield code="a">Creative Commons Attribution</subfield> </datafield> <datafield tag="024" ind1=" " ind2=" "> <subfield code="a">10.81043/aperta.93971</subfield> <subfield code="2">doi</subfield> </datafield> </record>
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