1 Ocak 2005 Dergi makalesi Açık Erişim

Bagis, H; Mercan, HO; Kumtepe, Y

Dublin Core

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  <dc:creator>Bagis, H</dc:creator>
  <dc:creator>Mercan, HO</dc:creator>
  <dc:creator>Kumtepe, Y</dc:creator>
  <dc:date>2005-01-01</dc:date>
  <dc:description>The objective of this study was to compare the effects of three different vitrification solutions on the development of pronuclear-stage (PN) Dinnyes mouse embryos into blastocyst stage after vitrification by solid surface vitrification (SSV) technique. For this aim, it was compared three experimental groups and a control group. Experimental groups were distinguished each other by the vitrification solution used in SSV. It was used 4% Ethylene Glycol (EG) at 37 degrees C equilibration temperature in the first group (SSV-EG), 4% Dimethyl Sulfoxide (DMSO) at room temperature in the second group (SSV-DMSO) and 4% Propylene Glycol (PG) at room temperature in the third group (SSV-PG). After vitrification, the survived embryos were cultured to blastocyst stage in KSOM. It was determined a significant difference between the groups of SSV-EG and SSV-PG at developing rate to 2-cell stage (P &lt; 0.05). Similarly, SSV-PG demonstrated significant differences with SSV-DMSO and the control group at developing rate to 3-8-cell stage (P &lt; 0.05). When compared the rates of developing to morula stage among the groups, it was determined significant differences between SSV-PG and the control group at (P &lt; 0.05); and between SSV-DMSO and SSV-PG (P &lt; 0.01). Finally, it was compared the developing rates into blastocyst stage and found that SSV-EG demonstrated significant differences with SSV-PG and SSV-DMSO (P &lt; 0.01). This study has shown that EG, DMSO and PG with trehalose can be used effectively as a cryoprotective agent in the quick freezing of pronuclear-stage mouse embryos. Finally, additional studies are needed to optimize the SSV method in further stage mouse embryos.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/93971</dc:identifier>
  <dc:identifier>oai:zenodo.org:93971</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>TURKISH JOURNAL OF VETERINARY &amp; ANIMAL SCIENCES 29(3) 621-627</dc:source>
  <dc:title>Effect of three different cryoprotectant solutions in solid surface vitrification (SSV) techniques on the development rate of vitrified pronuclear-stage mouse embryos</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
</oai_dc:dc>