1 Ocak 2005 Dergi makalesi Açık Erişim

Bagis, H; Mercan, HO; Kumtepe, Y

DataCite XML

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/93971</identifier>
  <creators>
    <creator>
      <creatorName>Bagis, H</creatorName>
      <givenName>H</givenName>
      <familyName>Bagis</familyName>
    </creator>
    <creator>
      <creatorName>Mercan, HO</creatorName>
      <givenName>HO</givenName>
      <familyName>Mercan</familyName>
    </creator>
    <creator>
      <creatorName>Kumtepe, Y</creatorName>
      <givenName>Y</givenName>
      <familyName>Kumtepe</familyName>
    </creator>
  </creators>
  <titles>
    <title>Effect Of Three Different Cryoprotectant Solutions In Solid Surface Vitrification (Ssv) Techniques On The Development Rate Of Vitrified Pronuclear-Stage Mouse Embryos</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2005</publicationYear>
  <dates>
    <date dateType="Issued">2005-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/93971</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.93970</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.81043/aperta.93971</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">The objective of this study was to compare the effects of three different vitrification solutions on the development of pronuclear-stage (PN) Dinnyes mouse embryos into blastocyst stage after vitrification by solid surface vitrification (SSV) technique. For this aim, it was compared three experimental groups and a control group. Experimental groups were distinguished each other by the vitrification solution used in SSV. It was used 4% Ethylene Glycol (EG) at 37 degrees C equilibration temperature in the first group (SSV-EG), 4% Dimethyl Sulfoxide (DMSO) at room temperature in the second group (SSV-DMSO) and 4% Propylene Glycol (PG) at room temperature in the third group (SSV-PG). After vitrification, the survived embryos were cultured to blastocyst stage in KSOM. It was determined a significant difference between the groups of SSV-EG and SSV-PG at developing rate to 2-cell stage (P &amp;lt; 0.05). Similarly, SSV-PG demonstrated significant differences with SSV-DMSO and the control group at developing rate to 3-8-cell stage (P &amp;lt; 0.05). When compared the rates of developing to morula stage among the groups, it was determined significant differences between SSV-PG and the control group at (P &amp;lt; 0.05); and between SSV-DMSO and SSV-PG (P &amp;lt; 0.01). Finally, it was compared the developing rates into blastocyst stage and found that SSV-EG demonstrated significant differences with SSV-PG and SSV-DMSO (P &amp;lt; 0.01). This study has shown that EG, DMSO and PG with trehalose can be used effectively as a cryoprotective agent in the quick freezing of pronuclear-stage mouse embryos. Finally, additional studies are needed to optimize the SSV method in further stage mouse embryos.</description>
  </descriptions>
</resource>