1 Ocak 2005 Dergi makalesi Açık Erişim

Bagis, H; Mercan, HO; Kumtepe, Y

JSON

{
  "conceptdoi": "10.81043/aperta.93970", 
  "conceptrecid": "93970", 
  "created": "2021-03-16T12:55:56.764727+00:00", 
  "doi": "10.81043/aperta.93971", 
  "files": [
    {
      "bucket": "373e2db9-2f04-479c-9a89-acaad52888de", 
      "checksum": "md5:9a7da9aa8f4d416219202f7e5b7aa85a", 
      "key": "bib-35255bf0-dc1d-4762-828b-4e099c29145b.txt", 
      "links": {
        "self": "https://aperta.ulakbim.gov.tr/api/files/373e2db9-2f04-479c-9a89-acaad52888de/bib-35255bf0-dc1d-4762-828b-4e099c29145b.txt"
      }, 
      "size": 276, 
      "type": "txt"
    }
  ], 
  "id": 93971, 
  "links": {
    "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.81043/aperta.93971.svg", 
    "bucket": "https://aperta.ulakbim.gov.tr/api/files/373e2db9-2f04-479c-9a89-acaad52888de", 
    "conceptbadge": "https://aperta.ulakbim.gov.tr/badge/doi/10.81043/aperta.93970.svg", 
    "conceptdoi": "https://doi.org/10.81043/aperta.93970", 
    "doi": "https://doi.org/10.81043/aperta.93971", 
    "html": "https://aperta.ulakbim.gov.tr/record/93971", 
    "latest": "https://aperta.ulakbim.gov.tr/api/records/93971", 
    "latest_html": "https://aperta.ulakbim.gov.tr/record/93971"
  }, 
  "metadata": {
    "access_right": "open", 
    "access_right_category": "success", 
    "communities": [
      {
        "id": "tubitak-adresli-yayinlar"
      }
    ], 
    "creators": [
      {
        "name": "Bagis, H"
      }, 
      {
        "name": "Mercan, HO"
      }, 
      {
        "name": "Kumtepe, Y"
      }
    ], 
    "description": "The objective of this study was to compare the effects of three different vitrification solutions on the development of pronuclear-stage (PN) Dinnyes mouse embryos into blastocyst stage after vitrification by solid surface vitrification (SSV) technique. For this aim, it was compared three experimental groups and a control group. Experimental groups were distinguished each other by the vitrification solution used in SSV. It was used 4% Ethylene Glycol (EG) at 37 degrees C equilibration temperature in the first group (SSV-EG), 4% Dimethyl Sulfoxide (DMSO) at room temperature in the second group (SSV-DMSO) and 4% Propylene Glycol (PG) at room temperature in the third group (SSV-PG). After vitrification, the survived embryos were cultured to blastocyst stage in KSOM. It was determined a significant difference between the groups of SSV-EG and SSV-PG at developing rate to 2-cell stage (P < 0.05). Similarly, SSV-PG demonstrated significant differences with SSV-DMSO and the control group at developing rate to 3-8-cell stage (P < 0.05). When compared the rates of developing to morula stage among the groups, it was determined significant differences between SSV-PG and the control group at (P < 0.05); and between SSV-DMSO and SSV-PG (P < 0.01). Finally, it was compared the developing rates into blastocyst stage and found that SSV-EG demonstrated significant differences with SSV-PG and SSV-DMSO (P < 0.01). This study has shown that EG, DMSO and PG with trehalose can be used effectively as a cryoprotective agent in the quick freezing of pronuclear-stage mouse embryos. Finally, additional studies are needed to optimize the SSV method in further stage mouse embryos.", 
    "doi": "10.81043/aperta.93971", 
    "has_grant": false, 
    "journal": {
      "issue": "3", 
      "pages": "621-627", 
      "title": "TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES", 
      "volume": "29"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2005-01-01", 
    "related_identifiers": [
      {
        "identifier": "10.81043/aperta.93970", 
        "relation": "isVersionOf", 
        "scheme": "doi"
      }
    ], 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "93971"
          }, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "93970"
          }
        }
      ]
    }, 
    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
    }, 
    "title": "Effect of three different cryoprotectant solutions in solid surface vitrification (SSV) techniques on the development rate of vitrified pronuclear-stage mouse embryos"
  }, 
  "owners": [
    1
  ], 
  "revision": 1, 
  "stats": {
    "downloads": 4.0, 
    "unique_downloads": 4.0, 
    "unique_views": 12.0, 
    "version_downloads": 4.0, 
    "version_unique_downloads": 4.0, 
    "version_unique_views": 12.0, 
    "version_views": 17.0, 
    "version_volume": 1104.0, 
    "views": 17.0, 
    "volume": 1104.0
  }, 
  "updated": "2021-03-16T12:55:56.814582+00:00"
}